Determination of Oligosaccharides in Ginseng from Different Growth Environments by Ultra Performance Liquid  Chromatography Triple Quadrupole Tandem Mass  Spectrometry Combined with Solid Phase Methylation

doi:10.19894/j.issn.1000-0518.200240

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (3): 247-255.DOI: 10.19894/j.issn.1000-0518.200240

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Determination of Oligosaccharides in Ginseng from Different Growth Environments by Ultra Performance Liquid Chromatography Triple Quadrupole Tandem Mass Spectrometry Combined with Solid Phase Methylation

ZHANG Na¹, LI Le-Le¹, HUANG Xin^1*, LIU Shu-Ying^1,2*

¹Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
²Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received:2020-08-12 Accepted:2020-10-14 Published:2021-03-01 Online:2021-05-01
Supported by:
National Key R&D Program of the Ministry of Science and Technology ( No.2017YFC1702105) and the Training Plan of Hundreds of Outstanding Treachers of Changchun University of Chinese Medicine (No.2017086)

Abstract

Abstract: The solid phase methylation column was packed with NaOH and CH₃I was used as the methylation reagent. The methylated oligosaccharides products were characterized by ultra performance liquid chromatography triple quadrupole tandem mass spectrometry (UPLC-QQQ-MS). The solid phase methylation conditions were further optimized based on previous work. Six reducing oligosaccharides (maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose and maltoheptaose) and three non-reducing oligosaccharides (sucrose, kestose and nystose) were detected in ginseng. This method could be applied in the quantitation of nine oligosaccharides in ginseng, simultaneously. UPLC-QQQ-MS combined with solid phase methylation could be used to efficiently derive and quantify oligosaccharides in ginseng. Therefore, an effective method is devdeloped to investigate the influence of growth environment on the distribution of oligosaccharides in ginseng.

Key words: Ultra performance liquid chromatography triple quadrupole tandem mass spectrometry, Solid phase methylation, Ginseng oligosaccharides, Growth environment

CLC Number:

O629.1

ZHANG Na, LI Le-Le, HUANG Xin, LIU Shu-Ying. Determination of Oligosaccharides in Ginseng from Different Growth Environments by Ultra Performance Liquid Chromatography Triple Quadrupole Tandem Mass Spectrometry Combined with Solid Phase Methylation[J]. Chinese Journal of Applied Chemistry, 2021, 38(3): 247-255.

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Determination of Oligosaccharides in Ginseng from Different Growth Environments by Ultra Performance Liquid Chromatography Triple Quadrupole Tandem Mass Spectrometry Combined with Solid Phase Methylation

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