Abstract:

The porous carbon-fiber/poly(lactic acid)/chitosan(CF/PLA/CS) composites scaffold was preparated by using solution blending and freeze-drying technique, and the biocompatibility between the composites and bone marrow stromal cells(BMSCs) was estimated by using inverted phase contrast microscope and scanning electron microscope(SEM). The curve of cell growth was obtained by using MTT assay, and the cell cytotoxicity were also assessed. The results showed that the cultured cells did not appear the deformation or death in the leach liquor and grew densely after 8 days. The SEM images showed that the cell morphology was normal, and the cell started to grow along the deep pore on the composites after 4 days. The MTT curve of cell growth indicated that cell proliferation rate of the experimental group was 30% higher than the blank test group after 4 days. According to the morphological observation and cell proliferation evaluation, it indicated the three-dimensional porous CF/PLA/CS composites do not have cell toxicity, and have excellent adhesion and proliferation of bone cells, suggesting that they may be promising bone repairing materials.

Key words: 3D, CF/PLA/CS Composites, In vitro, Cell compatibility