Chinese Journal of Applied Chemistry

Chinese Journal of Applied Chemistry

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Adsorption Behavior of Nicotinamide Adenine Dinucleotide on Nano-α-Aluminum Oxide

LI Li1,3, TANG Yongzheng1, JIANG Yuanyuan2, LU Tianhong2, YANG Xujie1, YANG Xiaodi1,2*   

  1. (1.Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education,
    School of Chemical Engineering,Nanjing University of Science and Technology,Nanjing 210094,China;
    2.Jiangsu Key Laboratory of New Power Batteries,College of Chemistry and
    Materials Science,Nanjing Normal University,Nanjing 210097,China;
    3.School of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University,Nanjing,China)
  • Received:2011-06-24 Revised:2011-08-29 Published:2012-05-10 Online:2012-05-10
  • Contact: Xiaodi

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Abstract: The adsorption behavior of nicotinamide adenine dinucleotide(NAD+) on α-Al2O3 nanoparticles was studied using ultraviolet-visible(UV-Vis) absorption spectroscopy, X-ray diffraction(XRD), fluorescence and attenuated total reflectance-Fourier transform infrared(ATR-FTIR) spectroscopy. It was found that the amount of adsorbed NAD+ exhibited a strong dependence on pH and ionic strength of the solution, which suggested that the adsorption of NAD+ at α-Al2O3 nanoparticles was caused predominantly by the electrostatic adsorption. ATR-FTIR spectra of adsorbed NAD+ were similar to those of NAD+ in solution. However, the position of peaks for phosphate moving to slightly higher wavenumbers suggested that phosphate participated into the electrostatic adsorption. The adsorption isotherm could be fitted with both Langmuir and Freundlich isotherms. The fluorescence spectral features showed that the conformation of NAD+ changed with the change of the amount of α-Al2O3 nanoparticles.

Key words: Adsorption, Nicotinamide adenine dinucleotide, Electrostatic interaction, Conformation

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