Abstract:

The fluorescence spectroscopic technique has been used to demonstrate the formation of the inclusion complex of adriamycin(ADM) and β-cyclodextrin(β-CD). The effects of the reaction time, concentration and temperature on the inclusion reaction are investigated. The chemical stoichiometric ratio for the formation of the inclusion complexes is determined. The inclusion constant and thermodynamic parameters of the inclusion reactions at different temperatures are calculated. At 33 ℃ and pH=7.0， the inclusion constant, K is 2.98×106 L/mol. The K value diminishes with the increase of temperature. Thus, the inclusion reaction is an enthalpy driving and spontaneous exothermic reaction. After ADM and β-CD form the inclusion complex, the fluorescence intensity is increased because β-CD can increase the natural fluorescence of ADM. Therefore, the fluorescence spectroscopic technique is an excellent technique for the investigation of the inclusion reaction of ADM and β-CD. The linear regression equation of the quantitative determination of ADM by β-CD sensitized fluorescence method is F=5.64×108c+47.26, with a correlation coefficient of 0.9985. The detection limit is 6.30×10-8 mol/L.

Key words: β-cyclodextrin, adriamycin, inclusion, fluorescence spectroscopy