Abstract:

SiO2/TiO2 hybrid materials were prepared via a sol-gel process followed with modification by bifunctional reagent of 3-(methoxy-silane-yl) propyl methacrylate(γ-MAPS). A new type of organic-inorganic hybrid capillary monolithic column was prepared in the capillary tube by in-situ polymerization of modified SiO2/TiO2 sol and poly-methyl-propyl-butyl acrylate(BMA). The microstructures of the bed-column cross-section were characterized by scanning electron microscopy(SEM). The column efficiency of 88000 plates per meter was obtained on the basis of evaluating the performance of the as-prepared monolithic column using thiourea as the electroosmotic flow(EOF) marker and investigating the stability and reproducibility of the column as well as the retention behavior of the neutral material on the column. The phosphorpeptide′s effective enrichment and separation were realized by the elution test of two kinds of short peptides(phosphoric acid peptide and non-phosphorylated peptides).

Key words: monolithic columns, capillary electrochromatography, sol-gel method, organic-inorganic hybrid materials, phosphopeptides