Abstract: With acrylamide-β-cyclodextrin(AM-β-CD) and glycidyl methacrylate acting as functional monomers, and ethylene glycol dimethacrylate acting as cross-linking agent, the chiral capillary monolithic column of acrylamide-β-cyclodextrin was prepared by in situ polymerization method. The structure and morphology of the monolithic column were characterized by IR and scanning electron microscopy(SEM). Meanwhile, the stability and reproducibility were also studied. The results showed that the capillary monolithic column of acrylamide-β-cyclodextrin had a good separation ability, and the column efficiency of 47701 plates/m was obtained. In capillary electrochromatography(CEC) mode, the etilefrine hydrochloride enantiomers were firstly separated by the chiral capillary monolithic column of acrylamide-β-cyclodextrin. Herein, the baseline resolutions of the enantiomers were completely reached.

Key words: Acrylamide-&, beta, -cyclodextrin, monolithic column, capillary electrochromatography, Etilefrine hydrochloride