Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (4): 546-553.DOI: 10.19894/j.issn.1000-0518.220357

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Effect of Electric Field-Regulating Cholesterol-based Liquid Crystal Films on the Growth and Differentiation of Fibroblasts

Yu-Chen TAO1, Xiao-Hui HOU1, Deng-Ke YIN1,3, Ye YANG1,2,3()   

  1. 1.School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China
    2.Anhui Key Laboratory of Technology and Application of Pharmaceutical Preparations,Hefei 230012,China
    3.Anhui Engineering and Technology Center for Modern Pharmaceutical Preparations,Hefei 230012,China
  • Received:2022-11-03 Accepted:2023-03-08 Published:2023-04-01 Online:2023-04-17
  • Contact: Ye YANG
  • About
  • Supported by:
    the National Natural Science Foundation of China(81874348);the Provincial Foundation for Discipline (Professional) Top Talent Talents of Colleges and Universities of Anhui Province of China(gxbjZD2022027);the Science and Technology Innovation Program of Drug Administration of Anhui(AHYJ-KJ-202110)


The liquid crystal ordering of cholesterol-g-polylactic acid oligomer (CLA) film is regulated by external direct-current electric field, and its effects on the adhesion, proliferation and differentiation of fibroblasts are evaluated. The liquid crystalline properties of CLA polymer aredetected by differential scanning calorimeter. The CLA films is prepared under a direct-current electric field with different intensities, and the liquid crystal ordering is detected by X-ray diffractometer and polarized light microscope. The adhesion and proliferation of fibroblasts on the CLA films are detected by a Cell Counting Kit-8 kit. The expression of α-smooth muscle actin (α-SMA) is detected by immunofluorescence staining and western blotting. The results show that CLA polymer has liquid crystal property, and the external electric field could regulate the liquid crystal property of CLA film through inducing the arrangement of CLA molecules. The enhancement of liquid crystal property of CLA films isbeneficial to the proliferation of fibroblasts and the expression of α?-SMA protein. These results suggest that electric field could promote the phenotypic transformation of fibroblasts to myofibroblasts by increasing the liquid crystal ordering of CLA polymer film, without changing the composition of matrix materials.

Key words: Liquid crystal, Polymeric material, Cholesteryl-oligo(L-lactic acid), Direct-current electric field, Fibroblasts, Phenotypic transformation

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