聚合物纳米组装体修饰丝网印刷电极构建过氧化氢传感器

doi:10.11944/j.issn.1000-0518.2020.02.190190

摘要/Abstract

摘要：

通过在聚合物结构中同时引入生物亲和单体和电活性单体,使得聚合物组装体在修饰丝网印刷电极时兼具提高比表面积、保持酶活和促进电子转移功能,从而发展了一种简单、高效构建电化学传感器的方法。以苯乙烯(St)、丙烯酸(AA)、N-乙烯基咔唑(VCz)和甲基丙烯酸二甲氨基乙酯(DMAEMA)为单体自由基聚合合成双亲性无规共聚物Poly(St-co-AA-co-VCz-co-DMAEMA)(PSACD),将聚合物在选择性混合溶剂DMF/H₂O中自组装得到聚合物纳米粒子PSACD NPs。利用纳米粒度分析仪、扫描电子显微镜(SEM)对其进行表征。依次将PSACD NPs水分散液、辣根过氧化物酶(HRP)溶液和全氟磺酸-聚四氟乙烯共聚物(Nafion,NF)溶液滴涂在丝网印刷碳电极(SPCE)上,制备得到过氧化氢生物传感器。通过计时电流法对传感器性能进行研究,表明该传感器对H₂O₂在0.02~7.48 mmol/L有良好的线性响应,且响应时间短(<2 s),具有良好的选择性和稳定性。

关键词: 聚合物纳米粒子, 自组装, 丝网印刷碳电极, 辣根过氧化物酶, 生物传感器

Abstract:

We report on a facile and efficient construction of electrochemical biosensors by modifying the screen printed carbon electrodes (SPCE) with multifunctional polymeric nanoparticles. An amphiphilic random copolymer, poly(St-co-AA-co-VCz-co-DMAEMA) (PSACD), was first synthesized using styrene (St), acrylic acid (AA), N-vinylcarbazole (VCz) and dimethylaminoethyl methacrylate (DMAEMA) as hydrophobic, hydrophilic, electroactive and enzymatic compatible monomers, respectively. The polymeric nanoparticles (PSACD NPs) were then prepared through self-assembly of polymers in a selective solvent mixture of DMF/H₂O. The obtained PSACD NPs were characterized by particle size analyzer and scanning electron microscope (SEM), and were used to fabricate the hydrogen peroxide (H₂O₂) biosensor with the functions of improving the specific surface areas, providing a suitable microenvironment for keeping the enzyme activity, and accelerating the electron transfer between enzymes and the electrodes. Specifically, the SPCE was successively modified by PSACD NPs suspensions, horseradish peroxidase (HRP) solution and perfluorosulfonic acid-PTFE copolymer (Nafion) solution. The properties of the proposed electrochemical biosensor were studied via an amperometric detection method. The results show that the biosensor has a short response time (less than 2 s) and a linear increasing response current with the concentration of H₂O₂ increasing from 0.02 to 7.48 mmol/L. The biosensor also has nice stability, good selectivity and excellent anti-interference performance.

Key words: polymeric nanoparticles, self-assembly, screen-printed carbon electrode, horseradish peroxidase, biosensor

黄雪雯, 许升, 赵伟, 魏玮, 李小杰, 刘晓亚. 聚合物纳米组装体修饰丝网印刷电极构建过氧化氢传感器[J]. 应用化学, 2020, 37(2): 235-241.

HUANG Xuewen, XU Sheng, ZHAO Wei, WEI Wei, LI Xiaojie, LIU Xiaoya. Hydrogen Peroxide Sensor Based on a Polymeric Self-assembled Nanoparticles-Modified Screen-Printed Electrode[J]. Chinese Journal of Applied Chemistry, 2020, 37(2): 235-241.

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