氮掺杂碳点的制备及其对阿莫西林高灵敏检测

doi:10.11944/j.issn.1000-0518.2020.02.190226

摘要/Abstract

摘要：

以天然物质石斛为原料,一步水热法合成高荧光量子产率的氮掺杂碳点(NCDs),通过透射电子显微镜(TEM)、X射线光电子能谱(XPS)、傅里叶变换红外光谱(FT-IR)、紫外-可见光吸收图谱(UV-Vis)及荧光光谱(PL)对合成的NCDs进行表征。实验结果显示合成的NCDs发强烈的蓝色荧光,呈现为球形或准球形,均匀分散,尺寸范围在1~5 nm;其表面含有丰富的COOH、OH和NH₂等水溶性基团,最佳激发和发射波长分别为350和435 nm,且具有良好的发光稳定性。通过测定,合成的NCDs的荧光量子产率高达29.19%。在pH=7.4的缓冲溶液中测定不同物质对NCDs的荧光影响,相同条件下发现只有阿莫西林能够对NCDs荧光进行明显猝灭,表明合成的NCDs可选择性的识别阿莫西林,通过NCDs的荧光强度变化构建一种可灵敏检测阿莫西林的传感器,检测线性范围为2.6~30 μmol/L,检出限为0.15 μmol/L。

关键词: 氮掺杂碳点, 高荧光量子产率, 荧光猝灭, 阿莫西林

Abstract:

In this paper, the nitrogen-doped carbon dots (NCDs) were synthesized using the natural material dendrobe as the raw material by one-step hydrothermal method. The synthesized carbon dots were characterized by transmission electron microscopy(TEM), X-ray photoelectron spectroscopy(XPS), Fourier transform infrared (FTIR) spectroscopy, ultraviolet-visible(UV-Vis) absorption spectroscopy and photoluminescence spectroscopy (PL). The experiment results show that NCDs are spherical or quasi-spherical, uniformly dispersed with the size ranges from 1 to 5 nm, which can emit strong blue fluorescence. The surface of the synthesized NCDs is rich in water-soluble groups such as COOH, OH and NH₂. The optimal excitation and emission wavelengths of NCDs are 350 and 435 nm, respectively. Meanwhile, the fluorescence emission has good luminescence stability. The fluorescence quantum yield of the carbon dots is as high as 29.19%. The effects of different substances on the fluorescence of NCDs were measured in a buffer solution with pH=7.4. Under the same conditions, only amoxicillin is able to significantly quench the fluorescence of NCDs, indicating the synthesized NCDs can selectively interact with amoxicillin. A sensitive sensor was constructed for detecting amoxicillin by changing the fluorescence intensity of carbon dots. The linear detection range for amoxicillin is from 2.6 to 30 μmol/L and the detection limit is 0.15 μmol/L.

Key words: nitrogen-doped carbon dots, high fluorescence quantum yield, fluorescence quenching, amoxicillin

弓辉, 康玉, 张荣, 任国栋, 侯笑雨, 张敏, 李丽红, 刘文, 王浩江, 刁海鹏. 氮掺杂碳点的制备及其对阿莫西林高灵敏检测[J]. 应用化学, 2020, 37(2): 227-234.

GONG Hui, KANG Yu, ZHANG Rong, REN Guodong, HOU Xiaoyu, ZHANG Min, LI Lihong, LIU Wen, WANG Haojiang, DIAO Haipeng. Preparation of Nitrogen-Doped Carbon Dots for Highly Sensitive Detection of Amoxicillin[J]. Chinese Journal of Applied Chemistry, 2020, 37(2): 227-234.

参考文献

[1]	De Baere S,De Backer P.Quantitative Determination of Amoxicillin in Animal Feed Using Liquid Chromatography with Tandem Mass Spectrometric Detection[J]. Anal Chim Acta,2007,586(1/2):319-325.
[2]	Bergamini M F,Teixeira M F S,Dockal E R,et al. Evaluation of Different Voltammetric Techniques in the Determination of Amoxicillin Using a Carbon Paste Electrode Modified with [N,N'-ethylenebis(salicylideneaminato)] Oxovanadium(IV)[J]. J Electrochem Soc,2006,153(5):E94-E98.
[3]	Shah K,Hassan E,Ahmed F,et al. Novel Fluorene-Based Supramolecular Sensor for Selective Detection of Amoxicillin in Water and Blood[J]. Ecotox Environ Safe,2017,141:25-29.
[4]	Cohen M L.Epidemiology of Drug Resistance:Implications for a Post-antimicrobial Era[J]. Science,1992,257(5073):1050-1055.
[5]	Gonzales R,Bartlett J G,Besser R E,et al. Principles of Appropriate Antibiotic Use for Treatment of Acute Respiratory Tract Infections in Adults:Background, Specific Aims, and Methods[J]. Ann Emerg Med,2001,37(6):690-697.
[6]	Shangguan J,Huang J,He D,et al. Highly Fe3+-Selective Fluorescent Nanoprobe Based on Ultrabright N/P Codoped Carbon Dots and Its Application in Biological Samples[J]. Anal Chem,2017,89(14):7477-7484.
[7]	Tan J,Zou R,Zhang J,et al. Large-scale Synthesis of N-Doped Carbon Quantum Dots and Their Phosphorescence Properties in a Polyurethane Matrix[J]. Nanoscale,2016,8(8):4742-4747.
[8]	Wang J,Qiu F,Wu H,et al. Fabrication of Fluorescent Carbon Dots-Linked Isophorone Diisocyanate and β-Cyclodextrin for Detection of Chromium Ions[J]. Spectrochim Acta A,2017,179:163-170.
[9]	HUANG Xiaomei,DENG Xiang.Preparation of New Photoluminescent Carbon Dots and Its Application in Hg2+ Detection[J]. Chinese J Appl Chem,2019,36(5):603-610(in Chinese). 黄小梅,邓祥. 新型荧光碳点的制备及其在Hg2+检测中的应用[J]. 应用化学,2019,36(5):603-610.
[10]	Luo M,Hua Y,Liang Y,et al. Synthesis of Novel β-cyclodextrin Functionalized S,N Codoped Carbon Dots for Selective Detection of Testosterone[J]. Biosens Bioelectron,2017,98:195-201.
[11]	WANG Shiqi,TU Yufei,LIU Zhixiao,et al. Microwave Synthesis of Nitrogen-Doped Carbon Dots and Its Application in Detection of Ferric Ions[J]. Chinese J Lumin,2019,40(6):751-757(in Chinese). 王诗琪,涂雨菲,刘之晓,等. 微波法制备掺氮碳点及用作探针检测铁离子[J]. 发光学报,2019,40(6):751-757.
[12]	Eda G,Lin Y Y,Mattevi C,et al. Blue Photoluminescence from Chemically Derived Graphene Oxide[J]. Adv Mater,2010,22(4):505-509.
[13]	Paredes J I,Villar-Rodil S,Martiínez-Alonso A,et al. Graphene Oxide Dispersions in Organic Solvents[J]. Langmuir,2008,24(19):10560-10564.
[14]	Zhu S,Meng Q,Wang L,et al. Highly Photoluminescent Carbon Dots for Multicolor Patterning, Sensors, and Bioimaging[J]. Angew Chem Int Ed,2013,52(14):3953-3957.
[15]	Wei J,Zhang X,Sheng Y,et al. Dual Functional Carbon Dots Derived from Cornflour via a Simple One-Pot Hydrothermal Route[J]. Mater Lett,2014,123:107-111.
[16]	Wu D,Huang X,Deng X,et al. Preparation of Photoluminescent Carbon Nanodots by Traditional Chinese Medicine and Application as a Probe for Hg2+[J]. Anal Meth,2013,5(12):3023-3027.
[17]	Gedda G,Lee C Y,Lin Y C,et al. Green Synthesis of Carbon Dots from Prawn Shells for Highly Selective and Sensitive Detection of Copper Ions[J]. Sens Actuators B-Chem,2016,224:396-403.
[18]	Wei J,Shen J,Zhang X,et al. Simple One-Step Synthesis of Water-Soluble Fluorescent Carbon Dots Derived from Paper Ash[J]. RSC Adv,2013,3(32):13119-13122.
[19]	Chen Y,Wu Y,Weng B,et al. Facile Synthesis of Nitrogen and Sulfur Co-doped Carbon Dots and Application for Fe(III) Ions Detection and Cell Imaging[J]. Sens Actuators B-Chem,2016,223:689-696