荧光素衍生物水杨醛荧光素腙的合成及对铜(Ⅱ)离子的检测

doi:10.11944/j.issn.1000-0518.2016.03.150238

摘要/Abstract

摘要：

以荧光素酰肼与5-溴水杨醛反应合成了一种新型荧光素衍生物5-溴水杨醛荧光素腙(BSFH),采用红外、核磁、质谱、元素分析等技术手段对其进行了表征。通过吸收光谱考察了在水溶液中BSFH对常见金属离子的选择性响应,发现BSFH在可见光区几乎无吸收,当加入常见金属离子时,吸收光谱上除了Cu²⁺之外的其它金属离子在496 nm处出现非常弱的吸收,而当Cu²⁺存在时,可以裸眼看到溶液颜色迅速从无色变为黄色,吸收光谱上在496 nm处出现了相对很强的吸收峰,并且随着Cu²⁺浓度的增加溶液的吸收强度不断增强,说明在水溶液中BSFH对Cu²⁺有很好的选择性。实验结果表明,该化合物与Cu²⁺的化学计量比为1:1,Cu²⁺浓度线性范围为0.30~10 μmol/L,许多常见的金属离子不干扰Cu²⁺的测定,检测限为0.30 μmol/L,说明在水溶液中BSFH对铜离子具有很高的灵敏度。据此,BSFH可以简单、快速、灵敏地在水溶液中识别和检测低浓度的铜离子。

关键词: 荧光素衍生物, 水杨醛荧光素腙, Cu²⁺, 吸收光谱, 检测

Abstract:

A novel fluorescein derivative, 5-bromosalicylicaldehyde fluorescein hydrazone(BSFH), has been synthesized by reacting fluorescein hydrazine with 5-bromosalicylicaldehyde and characterized by IR, NMR, MS, elemental analysis. The selectivity of BSFH to common metal ions in aqueous solution was investigated by absorption spectroscopy. BSFH has almost no absorption in visible region and very low absorption at 496 nm with addition of other metal ions except Cu²⁺. However, in the presence of Cu²⁺, a rapid color change of BSFH from colorless to yellow along with a very obvious absorption appears at 496 nm in the absorption spectrum. The absorbance of BSFH at 496 nm increases gradually with the increase of Cu²⁺. The selective binding of BSFH to Cu²⁺ over other metal ions is remarkably high in the aqueous solution. Experimental results indicate that the chemical stoichiometric ratio between BSFH and Cu²⁺ is 1:1, the linear range of detecting Cu²⁺ concentration is 0.30~10 μmol/L, common metal ions do not show any interference on the determination of Cu²⁺, the detection limit is 0.30 μmol/L, and BSFH is highly sensitive to Cu²⁺ in the aqueous solution. Therefore, BSFH can be utilized to detect Cu²⁺ at low concentration with simple, rapid and sensitive method.

Key words: fluorescein derivative, bromosalicylicaldehyde fluorescein hydrazone, cupper(Ⅱ) ion, absorption spectrum, detection

武鑫, 马骏, 南明, 双少敏, 董川. 荧光素衍生物水杨醛荧光素腙的合成及对铜(Ⅱ)离子的检测[J]. 应用化学, 2016, 33(3): 357-363.

WU Xin, MA Jun, NAN Ming, SHUANG Shaomin, DONG Chuan. Synthesis of a Fluorescein Derivative 5-Bromosalicylicaldehyde Fluorescein Hydrazone and the Detection for Copper(Ⅱ) Ion[J]. Chinese Journal of Applied Chemistry, 2016, 33(3): 357-363.

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