8-羟基脱氧鸟苷在壳聚糖/石墨烯修饰电极上的电化学及DNA氧化损伤检测

doi:10.3724/SP.J.1095.2014.30179

摘要/Abstract

摘要： 采用循环伏安(CV)、线性扫描伏安(LSV)和示差脉冲伏安(DPV)等方法研究了8-羟基脱氧鸟苷(8-OHdG)在壳聚糖(Chi)/石墨烯(GR)修饰的玻碳电极(GCE)上的电化学行为，8-OHdG在该修饰电极上氧化峰电流与其浓度在3.5×10-7~1.4×10-4 mol/L范围内呈良好的线性关系，检测限为6.4×10-8 mol/L(S/N=3)。将Chi/GR/GCE用于检测DNA氧化损伤，8-OHdG在修饰电极上的氧化峰电流与损伤的DNA质量浓度在10~300 mg/L范围内呈良好的线性关系，损伤DNA检出限为0.026 mg/L(S/N=3)。

关键词: 羟基脱氧鸟苷, 壳聚糖, 石墨烯, DNA氧化损伤, 电化学检测

Abstract: The electrochemical behavior of 8-hydroxydeoxyguanosine(8-OHdG) at the chitosan(Chi)/graphene(GR) modified glassy carbon electrode(GCE) was investigated by cyclic voltammetry, linear sweep voltammetry and differential pulse voltammetry. A linear relationship between the oxidative peak current and concentrations of 8-OHdG at the modified electrode was established in the range of 3.5×10-7~1.4×10-4 mol/L with a detection limit of 6.4×10-8 mol/L(S/N=3). Chi/GR/GCE was used for the detection of DNA oxidative damage, it showed that the oxidative peak current of 8-OHdG at the modified electrode was linear with concentration of damaged DNA in the range of 10~300 mg/L with a detection limit of 0.026 mg/L(S/N=3).

Key words: hydroxydeoxyguanosine, chitosan, graphene, DNA oxidative damage, electrochemical detection

中图分类号:

O657.1

安玲玲, 路毛凤, 邓玲玲, 杜江燕. 8-羟基脱氧鸟苷在壳聚糖/石墨烯修饰电极上的电化学及DNA氧化损伤检测[J]. 应用化学, DOI: 10.3724/SP.J.1095.2014.30179.

AN Lingling1, LU Maofeng1, DENG Lingling1, DU Jiangyan1,2,3*. Electrochemical Activities of 8-Hydroxydeoxyguanosine in Chitosan/Graphene Modified Glassy Carbon Electrode and Detection of DNA Oxidative Damage[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2014.30179.

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