应用化学

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石杉碱甲分子印迹聚合物的制备、表征及其吸附性能

李志平1,2,李辉2,3*,刘芬2,逯翠梅2   

  1. (1.吉首大学林产化工工程湖南省重点实验室 张家界 427000;2.吉首大学化学化工学院 吉首 416000;
    3.吉首大学生物资源与环境科学学院 吉首 416000)
  • 收稿日期:2012-10-19 修回日期:2013-02-15 出版日期:2013-08-10 发布日期:2013-08-10
  • 通讯作者: 李辉,教授; Tel/Fax:0743-8563911; E-mail:lihuijsdx@163.com; 研究方向:生命科学中的分离分析新方法
  • 基金资助:
    国家自然科学基金面上项目(21077042)林产化工工程湖南省重点实验室开放基金资助项目(JDZ201106)及湖南省教育厅重点项目(11A094)

Preparation, Characterization and Adsorption Behavior of Huperzine A Imprinted Polymers

LI Zhiping1,2, LI Hui2,3*, LIU Fen2, LU Cuimei2   

  1. (1.Key Laboratory of Hunan Forest Products and Chemical Industry Engineering,Jishou University,Zhangjiajie 427000,China;
    2.College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,China;
    3.College of Bio-resource and Environmental Science,Jishou University,Jishou 416000,China)
  • Received:2012-10-19 Revised:2013-02-15 Online:2013-08-10 Published:2013-08-10

摘要: 以硅胶为牺牲载体,石杉碱甲为模板分子,甲基丙烯酸为功能单体,二乙烯基苯为交联剂,偶氮二异丁腈为引发剂,首次制备了石杉碱甲分子印迹聚合物,并用红外光谱、扫描电子显微镜和热重分析研究了印迹聚合物的结构特征,用静态吸附法和Scatchard分析法研究了印迹聚合物的识别效能和表面位点分布特征。 结果表明,石杉碱甲印迹聚合物对模板分子具有较好的选择吸附性能,选择系数为1.399。Scatchard分析表明,印迹聚合物基体中主要存有两类吸附位点,对高亲和位点:平衡离解常数Kd1=0.776 g/L,最大表观结合量Qmax1=0.213 mg/g;对低亲和位点:平衡离解常数Kd2=0.169 g/L,最大表观结合量Qmax2=0.832 mg/g。 当该聚合物用于微固相萃取蛇足石杉粗提液中的石杉碱甲时,石杉碱甲回收率为93.5%,显示了较好的富集效果。

关键词: 分子印迹聚合物, 表面聚合, 石杉碱甲, 硅胶, 吸附

Abstract: Preparation of huperzine A molecularly imprinted polymers(MIPs) using silica as sacrificial material was carried out for the first time by using huperzine A as template molecule, methyl acrylic acid as functional monomer, divinyl benzene as cross-linker and 2′-2-azobis-isobutyronitrile as initiator, respectively. The MIPs were characterized by infrared spectroscopy(IR), scanning electron microscopy(SEM) and thermogravimetic analysis. Static method was employed to measure adsorption isotherm and Scatchard analysis was used to investigate on site distribution on the surface of MIPs. Results indicated that such MIPs exhibited a good selective adsorption performance with a selectivity coefficient of 1.399 toward the template. Two types of adsorption sites were mainly found in the matrix of the imprinted polymers with dissociation constants and apparent maximum binding capacity of 0.169 g/L and 0.832 mg/g for the low affinity sites, and 0.776 g/L and 0.213 mg/g for the high affinity ones. Additionally, a good enrichment capability toward huperzine A was also observed when this MIPs was used for solid phase micro-extraction of target compound from crude extract solution of Serrate Clubmoss. A recovery of 93.5% for the product could be achieved.

Key words: Molecularly imprinted polymers (MIPs), Surface imprinting, Huperzine A, Silica, Adsorption

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