中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (05): 531-536.DOI: 10.3724/SP.J.1095.2011.00418

• 研究论文 • 上一篇    下一篇

罗丹明B分子印迹聚合物微球的合成及其在固相萃取中的应用

雷秀兰1,2,易翔1,2,袁洋1,2,曾延波1,李蕾1*   

  1. (1.嘉兴学院生物与化学工程学院 嘉兴 314001;2.常州大学化学化工学院 常州 213016)
  • 收稿日期:2010-07-19 修回日期:2010-09-09 出版日期:2011-05-10 发布日期:2011-05-10
  • 通讯作者: 李蕾，教授; Tel/Fax:0573-83646280; E-mail:lileichem@yahoo.com.cn; 研究方向：分子识别与分离分析技术
  • 作者简介: 
  • 基金资助:
    浙江省科技计划重点(2007C23071)嘉兴市科技计划(2010AY1081)资助项目

Preparation of Rhodamine B Molecularly Imprinted Polymer Microspheres and Its Application in Solid Phase Extraction

LEI Xiulan1,2, YI Xiang1,2, YUAN Yang1,2, ZENG Yanbo1, LI Lei1*   

  1. (1.College of Biology and Chemical Engineering,Jiaxing University,Jiaxing 314001;
    2.College of Chemistry and Chemical Engineering,Changzhou University,Changzhou)
  • Received:2010-07-19 Revised:2010-09-09 Published:2011-05-10 Online:2011-05-10
  • Contact: li lei
  • About author: 

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1803

被引次数

11

摘要：

以罗丹明B为模板分子，丙烯酰胺为功能单体，乙二醇二甲基丙烯酸酯为交联剂，采用沉淀聚合法制备了罗丹明B分子印迹聚合物(MIP)微球，并用扫描电子显微镜表征。 采用紫外分光光度法测定了印迹分子罗丹明B与功能单体丙烯酰胺二者之间的结合常数(K=5.303×103 (mol/L)-1)和化学计量比(n=1)。 考察了沉淀剂的种类和用量对聚合物微球的影响。 将分子印迹聚合物微球应用于固相萃取材料自制固相萃取柱，从加标罗丹明B的红椒粉中萃取罗丹明B。 本文优化了固相萃取条件，高效液相色谱检测表明，在一定的萃取条件下，分子印迹聚合物对加标量为0.479 mg/kg的辣椒中罗丹明B的萃取加标回收率可达91.7％～103.5%。

关键词: 分子印迹聚合物微球, 罗丹明B, 沉淀聚合, 紫外光谱, 固相萃取

Abstract:

Molecular imprinting technology was employed to produce one kind of rhodamine B(RhB) molecularly imprinted polymer(MIP) microspheres by precipitation polymerization using RhB, acrylamide, ethylene glycol dimethacrylatea(EGDMA), azobisisobutyronitrile(AIBN), and acetonitrile as template, functional monomer, cross-linking agent, initiator and porogen, respectively. The resultant microspheres were characterized by scanning electron microscopy. The binding constant and chemical combination ratio of the complex formed between the template and the functional monomer were obtained by UV spectrophotometry, which showed that the RhB-monomer forms 1∶1 complexes and the binding constant is 5.3×103 L/mol. The effects of types and volumes of the porogen on the size and the uniformity of microspheres were also discussed. After packing the microspheres into column, it was used to absorb RhB from red pepper. The extraction conditions of molecular imprinting solid phase extraction(MISPE) column for RhB were optimized. Pepper samples spiked with RhB were extracted by MISPE column and analyzed by high performance liquid chromatography. The recoveries of MISPE column for RhB extraction were found to be 91.7%～103.5%, indicating the feasibility of the prepared MIPs for RhB extraction.

Key words: molecularly imprinted polymer microspheres, rhodamine B, precipitation polymerization, ultraviolet spectrophotometry, solid phase extraction

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