人参不同部位提取物体外抗氧化活性及成分差异

doi:10.19894/j.issn.1000-0518.210317

摘要/Abstract

摘要： 比较研究了人参不同部位提取物体外的抗氧化活性及化学成分差异,为合理开发利用人参资源提供理论依据。分别采用1,1-二苯基-2-三硝基苯肼自由基清除法(DPPH)、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺自由基清除法(ABTS)和铁离子还原法(FRAP)评价人参不同部位提取物的抗氧化活性,利用气相色谱-质谱联用技术(GC-MS)对人参不同部位乙酸乙酯提取物(EAE)中的化合物进行鉴别与比较。 DPPH、ABTS及FRAP实验结果表明人参茎叶EAE提取物的抗氧化效果最好。其中DPPH试验EAE的半数抑制浓度(IC₅₀)值为(0.66±0.02) g/L,阳性药-2,6-二叔丁基对甲基苯酚(BHT)的IC₅₀值为(0.26±0.02) g/L;ABTS试验EAE IC₅₀值为(0.54±0.26) g/L,阳性药BHT的 IC₅₀值为(0.15±0.04) g/L;FRAP试验EAE结果为(272.09±3.78) mg/L,阳性药BHT的值为(1319.37±3.80) mg/L。经GC-MS分析,分别从人参根、人参茎叶、人参花和人参种子EAE提取物中鉴别出20、27、14和14种成分,其中共有成分1种,为亚油酸。本研究为更好地合理开发与利用人参资源及精深加工抗氧化食品、化妆品产品提供了理论依据。

关键词: 人参提取物, 抗氧化活性, 化学成分, 成分差异

Abstract: In order to provide theoretical basis for rational exploitation and utilization of ginseng resources, the differences of active antioxidants and chemical components in extracts from different parts of ginseng were compared. 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) radical scavenging assay, 2,2'-biazo-bis-3-ethylbenzothiazoline-6-sulfone (ABTS) radical scavenging assay and ferric ion reduction assay (FRAP) were used to evaluate the antioxidant activities of different ginseng extracts. The compounds in ethyl acetate (EAE) extracts from different parts of Panax ginseng were identified and compared by gas chromatography-mass spectroscopy (GC-MS). The results of DPPH, ABTS and FRAP experiments show that the EAE extracts of ginseng stem and leaf have the best antioxidant effect. The half inhibition concentration (IC₅₀) value of DPPH is (0.66±0.02) g/L, and the IC₅₀ value of positive drug-2,6-BHT is (0.26±0.02) g/L. The IC₅₀ value of ABTS is (0.54±0.26) g/L, and the IC₅₀ value of BHT is (0.15±0.04) g/L. The EAE of FRAP test is (272.09±3.78) mg/L, and the positive BHT value is (1319.37±3.80) mg/L. By GC-MS analysis, 20, 27, 14 and 14 components are identified from the EAE extracts of ginseng root, ginseng folium, ginseng flower and ginseng seed, respectively, of which one common component is linoleic acid. This study provides a theoretical basis for better exploitation and utilization of ginseng resources and intensive processing of antioxidant food and cosmetic products.

Key words: Ginseng extract, Antioxidant activity, Chemical composition, Difference comparison

中图分类号:

O657.6
TQ658

张慧娥, 侯剑峰, 王经元, 朱爽, 杜连云, 叶萍, 魏琨, 陈长宝, 李光, 王恩鹏. 人参不同部位提取物体外抗氧化活性及成分差异[J]. 应用化学, 2021, 38(11): 1531-1540.

ZHANG Hui-E, HOU Jian-Feng, WANG Jing-Yuan, ZHU Shuang, DU Lian-Yun, YE Ping, WEI Kun, CHEN Chang-Bao, LI Guang, WANG En-Peng. A Differential Study on in vitro Antioxidant Activity and Extract Composition of Different Parts of Panax Ginseng[J]. Chinese Journal of Applied Chemistry, 2021, 38(11): 1531-1540.

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