具有过氧化物酶活性的Imm-Fe3+-IL的制备及用于比色法测定H2O2和葡萄糖

doi:10.11944/j.issn.1000-0518.2020.10.200064

摘要/Abstract

摘要： 通过溶胶-凝胶法制备的Imm-Fe³⁺-IL纳米材料具有类过氧化物酶的活性,能够催化过氧化氢(H₂O₂)快速氧化3,3′,5,5′-四甲基联苯胺(TMB)产生相应的颜色变化。稳态动力学分析表明,Imm-Fe³⁺-IL纳米材料遵循典型的Michaelis-Menten模型和乒乓机理。辣根过氧化物酶(HRP)相比,Imm-Fe³⁺-IL纳米材料纳米材料具有更强的亲和性。联合葡萄糖氧化酶建立了H₂O₂和葡萄糖的比色检测方法。结果显示:H₂O₂和葡萄糖的浓度与反应体系的吸光度呈良好的线性关系,H₂O₂的线性范围为1~200 μmol/L,葡萄糖的线性范围为10~200 μmol/L,最低检出限(LOD)分别为0.35和3.31 μmol/L。

关键词: 离子液体, 过氧化物酶, 比色检测, H₂O₂, 葡萄糖

Abstract: The Imm-Fe³⁺-IL nanomaterials prepared by the sol-gel method have peroxidase-like activity,which can catalyze the rapid oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) with hydrogen peroxide (H₂O₂) to produce corresponding color changes. The steady-state kinetic analysis shows that the catalytic kinetics of Imm-Fe³⁺-IL follows the typical Michaelis-Menten model, and the Ping-Pong mechanism. Compared with horseradish peroxidase (HRP), Imm-Fe³⁺-IL nanomaterials have stronger affinity. Combined with glucose oxidase, a colorimetric detection method for H₂O₂ and glucose is established. The results show that the concentration of H₂O₂ and glucose shows a good linear relationship with the absorbance of the reaction system. The linear range of H₂O₂ is 1~200 μmol/L, the linear range of glucose is 10~200 μmol/L, and the limits of detection (LOD) are 0.35 and 3.31 μmol/L for H₂O₂ and glucose, respectively.

Key words: ionic liquid, peroxidase-like, colorimetric detection, H₂O₂, glucose

喻昌木, 张荣, 卢小鸾, 杨敏, 彭黔荣. 具有过氧化物酶活性的Imm-Fe³⁺-IL的制备及用于比色法测定H₂O₂和葡萄糖[J]. 应用化学, 2020, 37(10): 1211-1220.

YU Changmu, ZHANG Rong, LU Xiaoluan, YANG Min, PENG Qianrong. Preparation of Imm-Fe³⁺-IL with Peroxidase Activity and Its Colorimetric Determination of H₂O₂ and Glucose[J]. Chinese Journal of Applied Chemistry, 2020, 37(10): 1211-1220.

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具有过氧化物酶活性的Imm-Fe³⁺-IL的制备及用于比色法测定H₂O₂和葡萄糖

Preparation of Imm-Fe³⁺-IL with Peroxidase Activity and Its Colorimetric Determination of H₂O₂ and Glucose

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