多巴胺改性海藻酸多孔支架的制备与性能

doi:10.11944/j.issn.1000-0518.2018.06.170173

摘要/Abstract

摘要：

生物大分子海藻酸(Alg)由于其安全、无毒、可生物降解等特性而被广泛应用于组织工程领域。受海洋贻贝蛋白结构的启发,多巴胺(DA)具有优异的粘附性能,在碱性水溶液条件下可发生氧化自聚形成聚多巴胺(PDA)。以Alg为基体,加入PDA纳米粒子复合,并通过冷冻干燥法制备得到Alg/PDA多孔支架材料。结果表明,Alg/PDA多孔支架材料具有较为规整的内部结构。改变Alg质量浓度,Alg/PDA支架材料的孔径可控制在60~120 μm之间,孔隙率可控于80%~88%。所得的支架材料具有适宜大小的孔径和孔隙率,结果表明支架材料对细胞无毒副作用。

关键词: 多巴胺, 海藻酸, 多孔支架材料

Abstract:

Alginate(Alg) is widely used in the field of tissue engineering because of its safe, non-toxic and biodegradable properties. Inspired by mussel adhesive protein, dopamine(DA) has excellent adhesion property and can self-oxidize to polydopamine(PDA) in weak basic buffer solution. Alg/PDA porous scaffolds were prepared by freeze-dry of Alg and PDA mixture. The results show that Alg/PDA porous scaffolds have relatively homogeneous internal structure. By changing the mass concentration of Alg, the pore size of the Alg/PDA complex scaffolds can be limited to 60~120 μm, and the porosity of the scaffolds can be controlled in the range of 80%~88% which is appropriate for the growth of bond cells. The biocompatibility results indicate that the scaffolds show low cytotoxicity.

Key words: dopamine, alginate, porous scaffold

章朱迎,陈静,俞佳蕾,赵倩,曹传昊,沈佳丽,施冬健. 多巴胺改性海藻酸多孔支架的制备与性能[J]. 应用化学, 2018, 35(6): 665-673.

ZHANG Zhuying,CHEN Jing,YU Jialei,ZHAO Qian,CAO Chuanhao,SHEN Jiali,SHI Dongjian. Preparation and Properties of Polydopamine and Alginate Porous Complex Scaffolds[J]. Chinese Journal of Applied Chemistry, 2018, 35(6): 665-673.

参考文献

[1]	WANG Yingjun,DU Chang,ZHAO Naru,et al. Biomimetic Artificial Bone Repair Materials:A Review[J]. South China Univ Technol(Nat Sci Ed),2012,40(10):51-58(in Chinese). 王迎军,杜昶,赵娜如,等. 仿生人工骨修复材料研究[J]. 华南理工大学学报(自然科学版),2012,40(10):51-58.
[2]	Murphy C M,Schindeler A,Gleeson J P,et al. A Collagen-Hydroxyapatite Scaffold Allows for Binding and Co-delivery of Recombinant Bone Morphogenetic Proteins and Bisphosphonates[J]. Acta Biomater,2014,10(5):2250-2258.
[3]	Yao X D,Hu Y,Cao B,et al. Effects of Surface Molecular Chirality on Adhesion and Differentiation of Stem Cells[J]. Biomaterials,2013,34(36):9001-9009.
[4]	Wang X,Ma J,Feng Q,et al. Skeletal Repair in Rabbits with Calcium Phosphate Cements Incorporated Phosphorylated Chitin[J]. Biomaterials,2002,23(23):4591-4600.
[5]	Xu W,Wang L,Ling Y,et al. Enhancement of Compressive Strength and Cytocompatibility Using Apatite Coated Hexagonal Mesoporous Silica/Poly(Lactic Acid-Glycolic Acid) Microsphere Scaffolds for Bone Tissue Engineering[J]. RSC Adv,2014,4(26):13495-13501.
[6]	Pawar S N,Edgar K J.Alginate Derivatization:A Review of Chemistry, Properties and Applications[J]. Biomaterials,2012,33(11):3279-3305.
[7]	Lee Y J,Shin D S,Kwon O W,et al. Preparation of Atacticpoly(Vinyl Alcohol)/Sodium Alginate Blend Nanowebs by Electrospinning[J]. J Appl Polym Sci,2007,106(2):1337-1342.
[8]	Ci S,Huynh T H,Louie L,et al. Molecular Mass Distribution of Sodium Alginate by High-Performance Size-Exclusion Chromatography[J]. J Chromatogr A,1999,864(2):199-210.
[9]	Braccini I,Pérez S.Molecular Basis of Ca2+-Induced Gelation in Alginates and Pectins:The Egg-Box Model Revisited[J]. Biomacromolecules,2001,2(4):1089-1096.
[10]	Sharma S,Sanpui P,Chattopadhyay A,et al. Fabrication of Antibacterial Silver Nanoparticle-Sodium Alginate Chitosan Composite Films[J]. RSC Adv,2012,2(13):5837-5843.
[11]	Wang W,Wang X,Feng Q L,et al. Sodium Alginate as a Scaffold Material for Hepatic Tissue Engineering[J]. J Bioact Compat Polym,2003,18(4): 249-257.
[12]	Florczyk S J,Kim D,Wood D,et al. Influence of Processing Parameters on Pore Structure of 3D Porous Chitosan Alginate Polyelectrolyte Complex Scaffolds[J]. J Biomed Mater Res A,2011,98(4):614-620.
[13]	Sun J,Xiao W,Tang Y,et al. Biomimetic Interpenetrating Polymer Network Hydrogels Based on Methacrylated Alginate and Collagen for 3D Pre-Osteoblast Spreading and Osteogenic Differentiation[J]. Soft Matter,2012,8(8):2398-2404.
[14]	Balakrishnan B,Joshi N,Jayakrishnan A,et al. Self-Crosslinked Oxidized Alginate/Gelatin Hydrogel as Injectable, Adhesive Biomimetic Scaffolds for Cartilage Regeneration[J]. Acta Biomater,2014,10(8):3650-3663.
[15]	Waite J H.Surface Chemistry:Mussel Power[J]. Nat Mater,2008,7(1):8-9.
[16]	Lee H,Dellatore S M,Miller W M,et al. Mussel-Inspired Surface Chemistry for Multifunctional Coatings[J]. Science,2007,318(5849):426-430.
[17]	Ju K,Lee Y,Lee S,et al. Bioinspired Polymerization of Dopamine to Generate Melanin-Like Nanoparticles Having an Excellent Free-Radical-Scavenging Property[J]. Biomacromolecules,2011,12(3):625-632.
[18]	Cui G,Dan N,Dan W.Preparation and Characterization of Novel Dopamine-based Bioadhesive Hydrogels[J]. Chem J Chinese Univ,2017,38(2):318-325.
[19]	Zhang S,Xu K,Darabi M A,et al. Mussel-Inspired Alginate Gel Promoting the Osteogenic Differentiation of Mesenchymal Stem Cells and Anti-Infection[J]. Mater Sci Eng C,2016,69:496-504.
[20]	Scognamiglio F,Travan A,Borgogna M,et al. Enhanced Bioadhesivity of Dopamine-functionalized Polysaccharidic Membranes for General Surgery Applications[J]. Acta Biomater,2016,44:232-242.
[21]	SHI Guixin,WANG Shenguo,BEI Jianzhong.Preparation of Porous Cell Scaffolds of Poly(L-lactic acid)and Poly(L-lactic-co-glycolic acid) and the Measurement of Their Porosities[J]. Funct Polym,2001,14(1):7-11(in Chinese). 石桂欣,王身国,贝建中. 聚乳酸与聚乳酸-羟基乙酸多孔细胞支架的制备及孔隙的表征[J]. 功能高分子学报,2001,14(1):7-11
[22]	Zhang R,Ma P X.Poly(α-Hydroxyl Acids)/Hydroxyapatite Porous Composites for Bone-Tissue Engineering.I.Preparation and Morphology[J]. J Biomed Mater Res,1999,44(4):446-455.
[23]	Kokubo T,Takadama H.How Useful is SBF in Predicting in vivo Bone Bioactivity?[J]. Biomaterials,2006,27(15):2907-2915.
[24]	Ratner B. D.Biomaterials Science:An Introduction to Materials in Medicine[M]. Amsterdam:Elsevier Academic Press,2004:218.
[25]	Chatterjea A,Der Stok J V,Danoux C,et al. Inflammatory Response and Bone Healing Capacity of Two Porous Calcium Phosphate Ceramics in Critical Size Cortical Bone Defects[J]. J Biomed Mater Res A,2014,102(5):1399-1407.
[26]	Chai F,Abdelkarim M,Laurent T,et al. Poly-Cyclodextrin Functionalized Porous Bioceramics for Local Chemotherapy and Anticancer Bone Reconstruction[J]. J Biomed Mater Res B,2014,102(6):1130-1139.
[27]	LIU Xinhui,ZHANG Xiqing,LIU Jinlian,et al. Celluarcompatibilty of the Nano-Hap/Collagen and Bone Marrow Stem Cell[J]. Chinese J Pediatr Surg,2005,26(4):203-206(in Chinese). 刘新晖,张锡庆,刘进炼,等. 纳米相羟基磷灰石胶原复合材料与人骨髓基质干细胞体外相容性的研究[J]. 中华小儿外科杂志,2005,26(4):203-206.