一种可再生高选择性的硫化氢荧光探针

doi:10.11944/j.issn.1000-0518.2018.12.180026

摘要/Abstract

摘要：

为了方便地检测环境样品中的硫化氢,利用香豆素酰肼肟配体构建了一个基于其铜配合物的可再生高选择性的硫化氢荧光探针(1-Cu²⁺)。顺磁性Cu²⁺的荧光猝灭作用使探针的荧光很弱。 Na₂S溶液的加入可显著增强其荧光,其它常见阴离子(F^-,Cl^-,Br^-,I^-,CO32-,HPO42-,H₂PO4-,NO2-,NO3-,SO42-,CH₃COO^-,N3-,S₂O32-,CN^-)对配合物探针的荧光影响很小,共存时也不会干扰探针对硫化氢的增强响应。 Cu²⁺的加入能够再生探针(1-Cu²⁺),通过依次加入Cu²⁺和S^2-,可重复地检测S^2-。该探针响应时间快(~5 s),在0.5~5.0 μmol/L的范围内对H₂S响应呈线性,检测限低至37 nmol/L。

关键词: 香豆素酰肼肟, 铜配合物, 硫化氢, 可再生荧光探针, Turn-on型

Abstract:

For expedient detection of H₂S in environmental samples, a renewable H₂S fluorescent probe(1-Cu²⁺) was constructed based on a complex formed by coumarin hydrazide oxime fluorescent ligand and a Cu²⁺. The probe displays very weak emission owing to quenching effect of Cu²⁺. The emission could be enhanced in the presence of S^2-, but no obvious changes in fluorescence intensity were observed upon the addition of other relevant anionic analytes(F^-, Cl^-, Br^-, I^-, CO32-, HPO42-, H₂PO4-, NO2-, NO3-, SO42-, CH₃COO^-, N3-, S₂O32-, CN^-). That is to say the probe system exhibits a remarkably selective fluorescence “ON” behavior exclusively with sulfide. The probe could be regenerated by the addition of Cu²⁺, and therefore the probe could be utilized repeatedly to sense sulfide anions with Cu²⁺ and S^2-. The detection limit of the fluorescent assay for sulfide is as low as ~37.0 nmol/L with a rapid response time(a few seconds), and the linear range is 0.5~4.0 μmol/L.

Key words: coumarin hydrazide oxime, Cu²⁺ complex, hydrogen sulfide, renewable fluorescent probe, turn-on type

尹正日. 一种可再生高选择性的硫化氢荧光探针[J]. 应用化学, 2018, 35(12): 1514-1520.

YIN Zhengri. A Renewable High Selective Fluorescent Probe for Hydrogen Sulfide[J]. Chinese Journal of Applied Chemistry, 2018, 35(12): 1514-1520.

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