联苯类比率型荧光探针检测水中硫化氢

doi:10.11944/j.issn.1000-0518.2017.04.160301

摘要/Abstract

摘要：

以叠氮基为识别基团,4.4'-联苯二甲酸为初始原料,合成了一种可用于H₂S检测的联苯类比率型荧光探针2-叠氮基-4,4'-联苯二甲酸乙酯(WN),并通过¹H NMR、¹³C NMR以及MS等技术手段对其结构进行了表征。以检测水溶液中硫化氢为目的,系统地研究了其荧光特性。研究结果表明,WN对硫化氢具有高选择性和灵敏度,且对生物硫醇(Cys,Gsh)、活性氧化物(H₂O₂, ClO^-)、各种阴阳离子(H₂PO4-,SO42-,Cl^-,HCO3-,CO32-,Mg²⁺,Zn²⁺,K⁺,Ca²⁺,Na⁺)有很强的抗干扰能力,在较宽的pH值范围内,仍然表现出良好的荧光性能,在1.1~350 μmol/L范围内,NaHS的浓度与荧光强度呈现良好的线性关系,相关系数R²=0.9943,检出限度为1.07×10^-6 mol/L。通过对3种不同水样的测试,表明探针WN在水体中H₂S的检测方面具有一定的应用意义。

关键词: 硫化氢, 叠氮基, 比率型荧光探针

Abstract:

A biphenyl-derived ratiometric fluorescent probe(WN) for the detection of H₂S was synthesized using 4,4'-biphenyl dicarboxylic acid as the raw material and characterized by ¹H NMR, ¹³C NMR, and MS. For the purpose of detection of H₂S in aqueous solution, its fluorescent properties had been systematically researched. The results show that probe WN exhibits good sensitivity and selectivity for detection of H₂S, response time is 12 min. WN has strong anti-interference ability to biological mercaptan(Cys, Gsh), active oxides(H₂O₂, ClO^-), various anions and cations(H₂PO4-, SO42-, Cl^-, HCO3-, CO32-, Mg²⁺, Zn²⁺, K⁺, Ca²⁺, Na⁺), and a good fluorescence response in a wide pH range, the fluorescence in tensity has good linear relation ships with H₂S in the range of 1.1~350 μmol/L(R²=0.9943), the detection limit of H₂S is estimated to be 1.07×10^-6 mol/L. The test for three different water samples shows that the probe WN has a certain application significance in the detection of H₂S in water.

Key words: hydrogen sulfide, azide group, ratiometric fluorescent probe

王宁, 周冬雪, 杨金闯, 安悦, 吕成伟. 联苯类比率型荧光探针检测水中硫化氢[J]. 应用化学, 2017, 34(4): 449-455.

WANG Ning, ZHOU Dongxue, YANG Jinchuang, AN Yue, LYU Chengwei. Determination of Hydrogen Sulfide in Water by Biphenyl Ratio Fluorescence Probe[J]. Chinese Journal of Applied Chemistry, 2017, 34(4): 449-455.

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