中华医学超声杂志

应用化学 ›› 2010, Vol. 27 ›› Issue (03): 363-366.DOI: 10.3724/SP.J.1095.2010.90180

• 研究论文 • 上一篇    下一篇

硅溶胶-凝胶包埋纳米金和酶的葡萄糖生物传感器

高盐生,王媛,狄俊伟*   

  1. (苏州大学化学化工学院 苏州 215123)
  • 收稿日期:2009-03-18 修回日期:2009-06-02 出版日期:2010-03-10 发布日期:2010-03-10
  • 通讯作者: 狄俊伟，男，教授; E-mail:djw@suda.edu.cn; 研究方向：电化学传感器及电分析化学
  • 基金资助:
    江苏省教育厅自然科学基金（07KJB150101）

Glucose biosensor based on gold nanoparticles and enzyme embedded in silica sol-gel network

GAO Yan-Sheng, WANG Yuan, DI Jun-Wei*   

  1. (College of Chemistry and Chemical Engineering,Suzhou University,Suzhou 215123)
  • Received:2009-03-18 Revised:2009-06-02 Published:2010-03-10 Online:2010-03-10

PDF

1315

被引次数

20 | 2

摘要：

采用溶胶-凝胶技术将金纳米粒子和葡萄糖氧化酶一次性固定于硅溶胶-凝胶的网络结构中，制备了葡萄糖生物电化学传感器并优化了传感器的制备条件。酶电极对葡萄糖具有良好的电化学响应，葡萄糖浓度在0.02~2.0 mmol/L范围内和催化电流呈线性关系，检出限为0.005 mmol/L。酶电极在4 ℃下贮存100 d后对葡萄糖的响应仅下降8%。该酶电极灵敏度高、响应快、稳定性好。

关键词: 金纳米粒子, 溶胶-凝胶, 葡萄糖, 电化学传感器

Abstract:

A novel method for fabrication of glucose electrochemical biosensor based on simultaneously embedding gold nanoparticles and glucose oxidase in silica sol-gel network on gold electrode surface was investigated. The preparation conditions were optimized. The enzyme electrode displayed an excellent electrocatalytic response to glucose. The glucose sensor had a linear range from 0.02 to 2.0 mmol/L and the detection limit of 0.005 mmol/L. It was found that the response current to glucose decreased about 8% after storage at 4℃ for 100 days. The biosensor exhibited high sensitivity, rapid response and good long-term stability.

Key words: gold nanoparticles, sol-gel, glucose, electrochemical biosensor

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