中华医学超声杂志

应用化学 ›› 2009, Vol. 26 ›› Issue (09): 1045-1049.

• 研究论文 • 上一篇    下一篇

灯盏花素与DNA相互作用的电化学及吸收光谱研究

郭英1,2,贝玉祥1,高云涛1   

  1. (1.云南民族大学化学与生物技术学院 昆明  650031;2.郧阳师范高等专科学校化学系 丹江口)
  • 收稿日期:2008-07-16 修回日期:2008-12-22 出版日期:2009-09-10 发布日期:2009-09-10
  • 通讯作者: 高云涛，男，教授; E-mail:yuntaogao@sohu.com; 研究方向：分离分析化学
  • 基金资助:
    云南省社会发展科技计划项目（2007B148M）

Electrochemical and UV-Vis Spectrcmetry Studies on Interaction Between Breviscapine and DNA

GUO Ying1,2, BEI Yu-Xiang1, GAO Yun-Tao1   

  1. (1.College of Chemistry and BioScience,Yunnan Nationalities University,Kunming 650031;
    2.Department of Chemistry,Yunyang Teachers College,Danjiangkou)
  • Received:2008-07-16 Revised:2008-12-22 Published:2009-09-10 Online:2009-09-10

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摘要：

用电化学方法研究了灯盏花素与DNA的相互作用，考察了扫速对灯盏花素与DNA相互作用的影响，实验表明，DNA存在使灯盏花素氧化峰的电位正移，灯盏花素的氧化峰峰电流减小，灯盏花素335 nm吸收光谱的吸收峰降低，呈减色效应，且出现2个等电吸收点，说明灯盏花素与DNA的相互作用以嵌插作用为主。双链DNA(dsDNA)与灯盏花素的结合能力大于DNA(ssDNA)，结合比为3:1，结合常数β为3.63×1013。

关键词: 灯盏花素, DNA, 循环伏安法, 吸收光谱, 相互作用

Abstract:

The interaction between Breviscapine and DNA was studied by cyclic voltammetry and UV-Vis spectrcmetry. The results indicated that with the addition of DNA, the oxidation peak currents of Breviscapine decreased and peak potentials also shifted positively, and the interaction between Breviscapine and dsDNA was stronger than that between Breviscapine and ssDNA.The results showed that Breviscapine react with DNA mainly in a mode of intercalation, which was comfirm by UV-Vis spectrcmetry.The combining ratio of Breviscapine to dsDNA was 3:1, with the combining constants 3.63×1013.

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