离子排斥色谱法同时测定葡萄糖醛酸和内酯及葡萄糖醛酸稳定性

doi:10.3724/SP.J.1095.2014.30299

摘要/Abstract

摘要： 建立了同时检测葡萄糖醛酸和葡萄糖醛酸内酯的离子排斥色谱法。采用Boston HC-B75 H+色谱柱进行分离，以5 mmol/L H2SO4水溶液为流动相，流速0.6 mL/min，柱温25 ℃，紫外检测波长220 nm；根据外标法定量，葡萄糖醛酸和内酯分别在10～1200 mg/L和5～600 mg/L范围内线性良好（r＞0.9999）；葡萄糖醛酸、葡萄糖醛酸内酯的最低检测限及定量限均分别为0.1和0.3 mg/L，精密度实验RSD分别为1.00%和0.35%，平均回收率分别为99.69%和98.21%。该检测方法简便、快速、灵敏，可用于葡萄糖醛酸和葡萄糖醛酸内酯的同时测定。温度、pH值和溶剂对葡萄糖醛酸稳定性有影响，可为葡萄糖醛酸的分析与制备研究提供参考依据。

关键词: 离子排斥色谱, 葡萄糖醛酸, 葡萄糖醛酸内酯, 稳定性

Abstract: An analytical method was established for the simultaneous determination of glucuronic acid and glucuronolactone using ion-exclusion chromatography. The separation was performed on a Boston HC-B75 H+ column at 25 ℃ by HPLC equipped with an ultraviolet detector at the wavelength of 220 nm. The mobile phase is 5 mmol/L sulfuric acid with a flow rate of 0.6 mL/min. The quantification was performed by an external standard. The linear ranges for glucuronic acid and glucuronolactone are 10～1200 mg/L and 5～600 mg/L(r＞0.9999), respectively. The detection and quantification limits for the two compounds are 0.1 mg/L and 0.3 mg/L, respectively. The standard deviations for glucuronic acid and glucuronolactone are 1.00% and 0.35%, respectively. The recovery ratios for glucuronic acid and glucuronolactone are 99.69% and 98.21%, respectively. This method is convenient, rapid, sensitive and suitable for the simultaneous determination of glucuronic acid and glucuronolactone. Temperature, pH and solvent affected the stability of glucuronic acid.

Key words: ion-exclusion chromatography, glucuronic acid, glucuronolactone, stability

中图分类号:

O657.7

范艳群, 许建中, 徐询, 刘亿婕, 许晨. 离子排斥色谱法同时测定葡萄糖醛酸和内酯及葡萄糖醛酸稳定性[J]. 应用化学, DOI: 10.3724/SP.J.1095.2014.30299.

FAN Yanqun1,2, XU Jianzhong2, XU Xun1,2, LIU Yijie3, XU Chen2*. Simultaneous Determination of Glucuronic Acid and Glucuronolactone Using Ion-exclusion Chromatography[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2014.30299.

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