2-硫代巴比妥酸修饰金纳米探针高灵敏比色检测三聚氰胺

doi:10.3724/SP.J.1095.2014.30249

摘要/Abstract

摘要： 以2-硫代巴比妥酸(TBA)修饰金纳米粒子为探针，TBA与三聚氰胺通过氢键作用诱导金纳米探针团聚，进而使金纳米胶体颜色由酒红色变为蓝色。实验优化得最佳反应条件为在乙酸缓冲溶液（pH=7.0）介质中，室温反应15 min。对不同浓度三聚氰胺进行检测时发现，在0.062~0.18 μmol/L和0.18~6.0 μmol/L之间，A660/A520吸收比率与三聚氰胺浓度呈现良好的线性关系，检出限为0.043 μmol/L。该方法用于检测牛奶样品中的三聚氰胺的加标回收率为102.8%～105.3%。

关键词: 三聚氰胺, 硫代巴比妥酸, 金纳米探针, 比色法

Abstract: A simple and rapid colorimetric method was developed for the detection of melamine using 2-thiobarbituric acid-modified gold nanoparticles(TBA-AuNPs) as probes. Melamine can induce the aggregation of TBA-AuNPs via the hydrogen-bonding interactions in acetic acid buffer solutions(pH=7), resulting in the color change from wine-red to blue within 15 min at room temperature. Under optimized conditions, the absorption ratio of the A660/A520 shows good linear relationship with the melamine concentration in the range of 0.062~0.18 μmol/L and 0.18~6.0 μmol/L, respectively, with the detection limit of 0.043 μmol/L. The recoveries of the method are within the range of 102.8%～105.3%. It provides a platform for rapid, selective and sensitive colorimetric detection of melamine in milk samples.

Key words: melamine, thiobarbituric acid, gold nanoparticles probes, colorimetric method

中图分类号:

O657.3

费瑶, 郭涵, 奚焕祥, 陈婉熠, 蔡琳熠, 王爱军, 冯九菊. 2-硫代巴比妥酸修饰金纳米探针高灵敏比色检测三聚氰胺[J]. 应用化学, DOI: 10.3724/SP.J.1095.2014.30249.

FEI Yao1, GUO Han2, XI Huanxiang1, CHEN Wanyi1, CAI Linyi1, WANG Aijun1, FENG Jiuju1,2*. Using 2-Thiobarbituric Acid-modified Gold Nanoparticles as Colorimetric Probes for Sensitive Detection of Melamine[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2014.30249.

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