中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (04): 448-453.DOI: 10.3724/SP.J.1095.2011.00395

• 研究论文 • 上一篇    下一篇

磺化改性聚四氟乙烯纤维固相萃取-高效液相色谱联用测定奶制品中的三聚氰胺

张毅1,韩晓燕2*,赵培莉1,郭炜毅2,张政朴3   

  1. (1.天津中医药大学实验教学部 天津;
    2.天津中医药大学中药学院，天津市中药化学与分析重点实验室 天津300193;
    3.南开大学高分子所，功能高分子材料教育部重点实验室 天津)
  • 收稿日期:2010-07-06 修回日期:2010-09-20 出版日期:2011-04-10 发布日期:2011-04-10
  • 通讯作者: 韩晓燕，副教授; Tel:022-59596282; Fax:022-59596279; E-mail:hanxiaoyan@tjutcm.edu.cn; 研究方向：功能高分子材料及应用
  • 作者简介:张毅，男，1978-3-25，硕士，实验师，研究方向：功能高分子材料及应用  Email: zhangyi@tjutcm.edu.cn

Adsorbent Preconcentration by PTFE-g-GMA-SO3H Fiber Coupled with HPLC for Determination of Trace Melamine in Milk Preparations

ZHANG Yi1, HAN Xiaoyan2*, ZHAO Peili1, GUO Weiyi2, ZHANG Zhengpu3   

  1. (1.Department of Experimental Teaching，Tianjin University of Traditional Chinese Medicine,Tianjin 300193;
    2.Institute of Chinese Medical,Tianjin Key Laboratory of Chemistry and Analysis of
    Chinese Material Medical,Tianjin University of Traditional Chinese Medicine,Tianjin 300193;
    3.Institute of Polymer Chemistry,The Education Ministry Key Laboratory of 
    [JZ]Function Polymeric Materials,Nankai University,Tianjin)
  • Received:2010-07-06 Revised:2010-09-20 Published:2011-04-10 Online:2011-04-10
  • Contact: han xiaoyan

PDF

1291

被引次数

9 | 3

摘要：

采用新型固相萃取材料磺化的甲基丙烯酸缩水甘油酯接枝聚四氟乙烯（PTFE-g-GMA-SO3H）纤维填充微柱预富集和流动注射(FI)与高效液相色谱(HPLC)联用测定样品中痕量的三聚氰胺。 建立了以该纤维作为吸附剂在线测定奶制品中三聚氰胺的新方法。 对三聚氰胺的富集与洗脱条件进行了优化，并得出三聚氰胺的分析特性：该方法对三聚氰胺的检出限为1.13×10-2 mg/L，富集倍数为300，RSD为7.6%（n=9，三聚氰胺质量浓度为0.2 mg/L）。 该方法应用于2种奶制品中的痕量三聚氰胺的测定，样品加标回收率分别为98%和102.5%。

关键词: 磺化的接枝甲基丙烯酸缩水甘油酯的聚四氟乙烯纤维, 在线固相萃取, 三聚氰胺

Abstract:

A preconcentration column packed with home-made sulfonated glycidyl methacrylate(GMA)-grafted-polytetrafluoroethylene(PTFE) polymer(PTFE-g-GMA-SO3H) fiber, coupled with high-performance liquid chromatography(HPLC) and flow injection, fiber was developed for trace melamine determination in the samples. The on-line determination method using the fiber as adsorbent for trace melamine in two milk sample was demonstrated. The conditions for preconcentration and elution of melamine were optimized. In addition, analytical performance of trace melamine determination was acquired in this article. The enrichment factor for melamine was 300 and the detection limits was 1.13×10-2 mg/L. The relative standard derivation was 7.6%（n=9） when the melamine concentration was 0.2 mg/L, and the recovery of samples was 98% and 102.5%, respectively.

Key words: sulfonated glycidylmethacrylate grafted polytetrafluoroethylene fiber, on line solid extration, melamine

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