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烟酰胺腺嘌呤二核苷酸在纳米α-氧化铝上的吸附作用

李丽1,3,汤勇铮1,蒋园园2,陆天虹2,杨绪杰1,杨小弟1,2*   

  1. (1.教育部软化学与功能材料重点实验室 南京理工大学化工学院 南京 210094；
    2.江苏省新型动力电池重点实验室，南京师范大学化学与材料科学学院 南京 210097；
    3.南京晓庄学院生物化工与环境工程学院 南京)
  • 收稿日期:2011-06-24 修回日期:2011-08-29 出版日期:2012-05-10 发布日期:2012-05-10
  • 通讯作者: 杨小弟，教授; Tel:025-83598648; Fax:025-85891767; E-mail:yangxiaodi@njnu.edu.cn; 研究方向：元素形态分析
  • 基金资助:
    国家自然科学基金（20875047,20873065）重点基金（20833006）和江苏高校优势学科建设工程资助项目

Adsorption Behavior of Nicotinamide Adenine Dinucleotide on Nano-α-Aluminum Oxide

LI Li1,3, TANG Yongzheng1, JIANG Yuanyuan2, LU Tianhong2, YANG Xujie1, YANG Xiaodi1,2*   

  1. (1.Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education,
    School of Chemical Engineering,Nanjing University of Science and Technology,Nanjing 210094,China;
    2.Jiangsu Key Laboratory of New Power Batteries,College of Chemistry and
    Materials Science,Nanjing Normal University,Nanjing 210097,China;
    3.School of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University,Nanjing,China)
  • Received:2011-06-24 Revised:2011-08-29 Published:2012-05-10 Online:2012-05-10
  • Contact: Xiaodi

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摘要： 用紫外-可见吸收光谱、X射线衍射、荧光光谱和衰减全反射傅里叶变换红外(ATR-FTIR）光谱等方法，研究了烟酰胺腺嘌呤二核苷酸(NAD+)在纳米α-Al2O3粒子上的吸附行为。 实验结果显示，NAD+的吸附量受pH值和离子强度影响较大，说明NAD+主要通过静电作用吸附在纳米α-Al2O3粒子上。 采用ATR-FTIR光谱分析了不同pH值溶液中及被吸附的NAD+，发现吸附后的NAD+与溶液中NAD+ 的ATR-FTIR光谱相似，但磷酸根的吸收峰向高波数位移，说明磷酸根参与了表面静电作用。 吸附过程符合Langmuir和Freundlich等温式。 荧光实验结果显示，随着吸附剂α-Al2O3用量的变化，NAD+构象也发生变化。

关键词: 吸附, 烟酰胺腺嘌呤二核苷酸, 静电作用, 构象

Abstract: The adsorption behavior of nicotinamide adenine dinucleotide(NAD+) on α-Al2O3 nanoparticles was studied using ultraviolet-visible(UV-Vis) absorption spectroscopy, X-ray diffraction(XRD), fluorescence and attenuated total reflectance-Fourier transform infrared(ATR-FTIR) spectroscopy. It was found that the amount of adsorbed NAD+ exhibited a strong dependence on pH and ionic strength of the solution, which suggested that the adsorption of NAD+ at α-Al2O3 nanoparticles was caused predominantly by the electrostatic adsorption. ATR-FTIR spectra of adsorbed NAD+ were similar to those of NAD+ in solution. However, the position of peaks for phosphate moving to slightly higher wavenumbers suggested that phosphate participated into the electrostatic adsorption. The adsorption isotherm could be fitted with both Langmuir and Freundlich isotherms. The fluorescence spectral features showed that the conformation of NAD+ changed with the change of the amount of α-Al2O3 nanoparticles.

Key words: Adsorption, Nicotinamide adenine dinucleotide, Electrostatic interaction, Conformation

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