Study on Extract Composition of American Ginseng Flower in Oxidative-Induced H9c2 Cardromyocytes by LC-MS

doi:10.19894/j.issn.1000-0518.220131

Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (1): 126-133.DOI: 10.19894/j.issn.1000-0518.220131

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Study on Extract Composition of American Ginseng Flower in Oxidative-Induced H9c2 Cardromyocytes by LC-MS

Jun-Jie ZHANG¹, Yun-Jiao SHEN¹, Li-Ying MA¹, Peng-Hui WANG¹, Lei WANG¹, Yu-Lin DAI²(), Lei ZHAO¹()

^1.School of Pharmaceutical Sciences，Changchun University of Chinese Medicine，Changchun 130117，China
^2.Jilin Ginseng Academy，Changchun University of Chinese Medicine，Changchun 130117，China

Received:2022-04-13 Accepted:2022-09-21 Published:2023-01-01 Online:2023-01-28
Contact: Yu-Lin DAI,Lei ZHAO
About author:zhaolei@ccucm.edu.cn
dyltcm@163.com；
Supported by:
the Jilin Province Science and Technology Development Plan(2200404038YY)

Abstract

Abstract:

In this study， the American ginseng flower is pre-treated by high temperature and pressure and the ethanol extract of American ginseng flower is divided by n-hexane， chloroform， ethyl acetate and n-butanol consequently. A total of 10 different polar components are obtained. The best active fraction is screened by oxidative-induced H9c2 Cardromyocytes. The ability of free radical scavenging and chemical composition of the fractions are analyzed by LC-MS. The results show that the processed ethyl acetate extraction （PEE） of American ginseng flowers effectively protects oxidative-induced H9c2 Cardromyocytes， significantly improves cell viability and reduces cell ROS levels. Compared with the original ethyl acetate extraction （OEE）， the ginsenoside content of the two groups is similar and shows strong antioxidant capacity. Through LC-MS analysis， it is preliminarily determined that Rk₁ and Rg₅ generated by high temperature and pressure transformation in PEE. Therefore， processed American ginseng flower has better protective effect on oxidative-induced H9c2 Cardromyocytes than original.

Key words: American ginseng flower, Myocardial protection, Non-medicinal part, Ginsenosides, Antioxidation, LC-MS

CLC Number:

O629

Jun-Jie ZHANG, Yun-Jiao SHEN, Li-Ying MA, Peng-Hui WANG, Lei WANG, Yu-Lin DAI, Lei ZHAO. Study on Extract Composition of American Ginseng Flower in Oxidative-Induced H9c2 Cardromyocytes by LC-MS[J]. Chinese Journal of Applied Chemistry, 2023, 40(1): 126-133.

Figures/Tables 7

References 26

1	张正海，雷慧霞，钱佳奇，等. 西洋参的引种简史［J］. 人参研究， 2020， 32（2）： 59-62.
	ZHANG Z H， LEI H M， QIAN J Q， et al. Introduction history of Panax quinquefolium L.［J］. Ginseng Res， 2020， 32（2）： 59-62.
2	尹红新. 西洋参花药及原生质体培养研究［D］. 北京：中国农业科学院， 2015.
	YIN H X. Research on anther and protoplast culture of Panax quinquefolius L.［D］. Beijing： Chinese Academy of Agricltural Sciences， 2015.
3	孟祥颖，李向高，于洋. 国产西洋参花蕾化学成分的研究Ⅰ.人参皂苷的分离、鉴定及含量测定［J］. 吉林农业大学学报， 2000（3）： 1-8.
	MENG X Y， LI X G， YU Y. Chemical constituents of flowerbuds of Panax quinquefolium L. culticated in ChinaⅠ. isolation， indentification and content determination of saponins［J］. J Jilin Agric Univ， 2000（3）： 1-8.
4	赵雷. 中药多糖类物质的应用研究与现状分析［J］. 长春中医药大学学报， 2006， 22（3）： 67-68.
	ZHAO L. Application research and cstatus analysis of polysaccharides in traditional Chinese medicine［J］. J Changchun Univ Trad Chin Med， 2006， 22（3）： 67-68.
5	刘雪莹，赵雨，刘莉，等. 西洋参花多糖的提取和体外免疫调节作用的研究［J］. 食品工业， 2018， 39（1）： 23-25.
	LIU X Y， ZHAO Y， LIU L， et al. The extraction of polysaccharides from American ginseng flower and immunomodulation activities in vitro［J］. Food Ind， 2018， 39（1）： 23-25.
6	马晓宁，李巍，赵余庆. 西洋参花蕾总皂苷水解产物中稀有抗肿瘤成分的化学研究［J］. 中草药， 2008， 39（9）： 1291-1294.
	MA X N， LI W， ZHAO Y Q. Rare constituents with anti-cancer activity from hydrolytic products in saponins of Panax quinquefolium stems and leaves［J］. Chin Trad Herbal Drugs， 2008， 39（9）： 1291-1294.
7	YU J， XU T， LIN H， et al. Comprehensive quality evaluation of American ginseng for different parts and abnormal trait based on the major ginsenoside contents and morphological characteristics［J］. Biomed Res Int， 2021， 2021：8831080.
8	李乐，谭璐瑩，王彩霞，等. 超高效液相色谱-四极杆飞行时间质谱法鉴定西洋参果梗化学成分［J］. 应用化学， 2021， 38（3）： 256-270.
	LI L， TAN L Y， WANG C X， et al. Identification of chemical constituents from American ginseng fruit pedicels by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry［J］. Chin J Appl Chem， 2021， 38（3）： 256-270.
9	李丽，刘春明，吴巍，等. 高效液相色谱-电喷雾质谱联用法测定人参和西洋参的皂苷类成分［J］. 分析化学， 2005， 33（8）： 1087-1090.
	LI L， LIU C M， WU W， et al. Analysis of saponins in extract of Panax ginseng and Panax quinquofolium by liquid chromatography-electrospray ionization mass spectrometry［J］. Chin J Anal Chem， 2005， 33（8）： 1087-1090.
10	BECCARIA M， CABOOTER D. Current developments in LC-MS for pharmaceutical analysis［J］. Analyst， 2020， 145（4）： 1129-1157.
11	张语迟，刘箬瑶，刘春明，等. 利用液质联用技术分析高温处理对人参皂苷的转化规律［J］. 长春师范大学学报， 2022， 41（2）： 70-74.
	ZHANG Y C， LIU R Y， LIU C M， et al. Study on the ginsenosides transform regulation of steam ginseng with high temperature by HPLC-ESI-MS［J］. J Changchun Norm Univ， 2022， 41（2）： 70-74.
12	DAI Y L， ZHOU D Y， JIANG Y F， et al. 6-Bromohypaphorine isolated from red sea cucumbers Apostichopus japonicus exhibits potent anticancer activity in A549 cancer cell line［J］. Chin J Anal Chem， 2021， 49（12）： 43-48.
13	ERUSLANOV E， KUSMARTSEV S. Identification of ROS using oxidized DCFDA and flow-cytometry［J］. Methods Mol Biol， 2010， 594： 57-72.
14	叶丽华，叶倩灵，章晓红，等. 优化参麦注射液人参总皂苷含量测定方法［J］. 药学研究， 2017， 36（4）： 203-205， 242.
	YE L H， YE Q L， ZHANG X H， et al. Optimization method for the determination of total ginsenosides in Shenmai injection［J］. Chin J Pharm Res， 2017， 36（4）： 203-205， 242.
15	旷春桃，李湘洲，汪玉霞，等. 大叶冬青叶中总黄酮测定方法和提取工艺研究［J］. 食品科学， 2009， 30（6）： 49-51.
	KUANG C T， LI X Z， WANG Y X， er al. Study on determination methods and extraction process of total flavonoids in leaves of Ilex latifolia［J］. Chin J Food Sci， 2009， 30（6）： 49-51.
16	HUANG F Q， DONG X， YIN X， et al. A mass spectrometry database for identification of saponins in plants［J］. J Chromatogr A， 2020， 1625： 461296.
17	戴雨霖，越皓，孙长江，等. 高分离度快速液相色谱-四极杆-飞行时间质谱法分析酒制和醋制人参的皂苷类成分［J］. 分析化学， 2015， 43（8）： 1181-1186.
	DAI Y L， YUE H， SUN C J， et al. Determination of ginsenosides in processed ginseng by rapid resolution liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry［J］. Chin J Anal Chem， 2015， 43（8）： 1181-1186.
18	乔梦丹，刘尚，张琰，等. 基于UPLC-Q-Orbitrap MS/MS研究人参皂苷在发酵过程中的生物转化［J］. 高等学校化学学报， 2018， 39（2）： 219-225.
	QIAO M D， LIU S， ZHANG Y， et al. Biotransformation of ginsenosides in fermented ginseng using UPLC-Q-Orbitrap MS/MS［J］. Chem J Chin Univ， 2018， 39（2）： 219-225.
19	黄鑫，王妮，张勇，等. 超高效液相色谱-四极杆静电场轨道阱高分辨质谱联用法分析不同西洋参加工品的皂苷类成分［J］. 分析测试学报， 2018， 37（6）： 646-652.
	HUANG X， WANG N， ZHANG Y， et al. Analysis of ginsenosides in different processed Panax quinquefoliums L. roots by UPLC Orbitrap HRMS［J］. Chin J Instru Anal， 2018， 37（6）： 646-652.
20	关大朋，王欢，李伟，等. 高温热裂解人参皂苷Rk₁和Rg₅的制备工艺优化［J］. 上海中医药杂志， 2015， 49（1）： 91-95.
	GUANG D P， WANG H， LI W， et al. Optimization of preparing technology of ginsenoside Rk₁ and Rg₅ by high-temperature pyrolysis［J］. Shanghai J Trad Chin Med， 2015， 49（1）： 91-95.
21	张楠淇. PF₁₁滴丸抗心肌缺血作用及其药代动力学研究［D］. 长春：吉林大学， 2018.
	ZHANG N Q. Study on the anti-myocardial ischemia effect and pharmacokinetics of PF₁₁ pills［D］. Changchun： Jilin University， 2018.
22	刘月阳，高永峰，杨静玉，等. 拟人参皂苷-F₁₁对大鼠血栓栓塞性脑卒中的防治作用及血栓性炎症相关调控机制［J］. 中国药理学与毒理学杂志， 2021， 35（10）： 792.
	LIU Y Y， GAO Y F， YANG J Y， et al. The preventive effect of pseudoginsenoside-F₁₁ on thromboembolic stroke in rats and the related regulatory mechanism of thrombotic inflammation［J］. Chin J Pharm Toxicio， 2021， 35（10）： 792.
23	赵翔宇，何振宇，宰守峰. 人参皂苷Rg₅对胃癌细胞周期和侵袭的影响及其机制［J］. 中国应用生理学杂志， 2020， 36（1）： 51-55.
	ZHAO X Y， HE Z Y， ZAI S F. Effects of ginsenoside Rg₅ on cell cycle and invasion of gastric cancer［J］. Chin J Appl Physiol， 2020， 36（1）： 51-55.
24	郭慧，刘鹏飞. 人参皂苷Rg₅通过抑制蛋白激酶B信号通路调控肝癌HepG2细胞生物学行为［J］. 安徽医药， 2021， 25（6）： 1095-1099， 1275.
	GUO H， LIU P F. Ginsenoside Rg₅ regulates the biological behavior of hepatocellular carcinoma HepG2 cells by inhibiting AKT signaling pathway［J］. Anhui Med Pharm J， 2021， 25（6）： 1095-1099， 1275.
25	叶安琪，张仔豪，成乐琴. 人参皂苷Rg₅的研究进展［J］. 沈阳药科大学学报， 2020， 37（12）： 1144-1152.
	YE A Q， ZHANG Z H， CHENG Y Q. Research progress of ginsenoside Rg₅［J］. J Shenyang Pharm Univ， 2020， 37（12）： 1144-1152.
26	曾露露，丁传波，刘文丛，等. 人参稀有皂苷药理活性的研究进展［J］. 时珍国医国药， 2018， 29（3）： 680-682.
	ZENG L L， DING C B， LIU W C， et al. Research progress on the pharmacological activity of rare ginsenoside［J］. Lishizhen Med Mater Med Res， 2018， 29（3）： 680-682.

Ingredient content	w（American ginseng flower）/%	w（Original ethyl acetate extracts）/%	w（Processed ethyl acetate extracts）/%
Moisture	9.37±0.03	—	—
Total ash	6.19±0.04	—	—
Total ginsenosides	—	43.26±0.21	43.57±0.29
Total protein	—	6.32±0.16	5.09±0.07
Total flavone	—	2.74±0.20	2.05±0.22

Ingredient content	w（American ginseng flower）/%	w（Original ethyl acetate extracts）/%	w（Processed ethyl acetate extracts）/%
Moisture	9.37±0.03	—	—
Total ash	6.19±0.04	—	—
Total ginsenosides	—	43.26±0.21	43.57±0.29
Total protein	—	6.32±0.16	5.09±0.07
Total flavone	—	2.74±0.20	2.05±0.22

No.	t_R/min	Compound	Found mass	Theoretical mass	Mass error/ （×10^-6）	OEE/%	PEE/%
1	12.96	Rg₁	845.492 1	845.490 3	-1.8	0.31	0.17
2	13.44	Re	991.550 3	991.548 2	-2.1	0.74	0.47
3	14.54	p-F₁₁	799.480 5	799.484 4	-3.9	0.95	1.6
4	18.39	Unknown	843.476 1	—	—	0.22	0.57
5	18.81	Rf	845.491 8	845.490 4	-1.4	48.32	12.91
6	19.79	Unknown	843.476 8	—	—	0.23	0.82
7	21.07	20（S）-Rg₂	829.497 4	829.495 9	-1.5	14.9	8.18
8	21.59	20（R）-Rg₂	829.497 7	829.495 8	-1.9	0.18	1.94
9	37.88	Rk₁	811.486 3	811.485 1	-1.2	0.18	3.2
10	38.16	Unknown	843.475 9	—	—	0.02	0.88
11	38.94	Rg₅	811.486 5	811.485 1	-1.4	0.29	3.18

No.	t_R/min	Compound	Found mass	Theoretical mass	Mass error/ （×10^-6）	OEE/%	PEE/%
1	12.96	Rg₁	845.492 1	845.490 3	-1.8	0.31	0.17
2	13.44	Re	991.550 3	991.548 2	-2.1	0.74	0.47
3	14.54	p-F₁₁	799.480 5	799.484 4	-3.9	0.95	1.6
4	18.39	Unknown	843.476 1	—	—	0.22	0.57
5	18.81	Rf	845.491 8	845.490 4	-1.4	48.32	12.91
6	19.79	Unknown	843.476 8	—	—	0.23	0.82
7	21.07	20（S）-Rg₂	829.497 4	829.495 9	-1.5	14.9	8.18
8	21.59	20（R）-Rg₂	829.497 7	829.495 8	-1.9	0.18	1.94
9	37.88	Rk₁	811.486 3	811.485 1	-1.2	0.18	3.2
10	38.16	Unknown	843.475 9	—	—	0.02	0.88
11	38.94	Rg₅	811.486 5	811.485 1	-1.4	0.29	3.18

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Study on Extract Composition of American Ginseng Flower in Oxidative-Induced H9c2 Cardromyocytes by LC-MS

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