Preparation of Lysozyme-Responsive Nanodiamond Drug-Loading System and the Effect on HepG2 Cells

doi:10.11944/j.issn.1000-0518.2019.10.190112

Chinese Journal of Applied Chemistry ›› 2019, Vol. 36 ›› Issue (10): 1109-1117.DOI: 10.11944/j.issn.1000-0518.2019.10.190112

• Full Papers • Previous Articles Next Articles

Preparation of Lysozyme-Responsive Nanodiamond Drug-Loading System and the Effect on HepG2 Cells

LI Lin^a^b^*(),SONG Jixiang^a,LI Yingqi^c,ZHANG Caifeng^a^b

^aDepartment of Chemistry,Taiyuan Normal University,Jinzhong,Shanxi 030619,China
^bHumic Acid Engineering and Technology Research Center of Shanxi Province,Jinzhong,Shanxi 030619,China
^cDepartment of Chemistry,College of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China

Received:2019-04-16 Accepted:2019-08-19 Published:2019-10-01 Online:2019-09-29
Contact: LI Lin
Supported by:
Supported by Shanxi Youth Science and Technology Research Fund Project(No.201801D221440), the Taiyuan Normal University College Students Science and Technology Innovation Project(No.2018503 CXCY1850), and the Shanxi Provincial Applied Fundamental Research Fund Project(No.201801D121257)

Abstract

Abstract:

Glycidol(GLY) and doxorubicin(DOX) were coupled to ACAylated nanodiamond(ND) carrier to obtain nanodiamond-aminocaproic acid-glycidol-doxorubicin (ND-ACA-GLY-DOX, NAGD) drug delivery system through ester bond. The amounts of ACA and DOX coupled to the surface of the ND were (185±10.0) μg/mg and (115±5.2) μg/mg, respectively, as determined by fluorescence spectroscopy. The results showed that the amount of NAGD released was very low in the physiological environment(pH=7.4), while the ester bond was hydrolytically cleaved and a large amount of DOX was released in the presence of lysozyme in the lysosome environment of the tumor cells. Using liver cancer cells as the model, the cell morphology test showed that NAGD could effectively kill tumor cells. The above results indicate that NAGD can be used as a good drug delivery system.

Key words: nanodiamond, aminocaproic acid, glycidol, doxorubicin, lysozyme responsive

LI Lin, SONG Jixiang, LI Yingqi, ZHANG Caifeng. Preparation of Lysozyme-Responsive Nanodiamond Drug-Loading System and the Effect on HepG2 Cells[J]. Chinese Journal of Applied Chemistry, 2019, 36(10): 1109-1117.

References

[1]	GolobSchwarzl N,Krassnig S,Toeglhofer A M,et al. New Liver Cancer Biomarkers: PI3K/AKT/mTOR Pathway Members and Eukaryotic Translation Initiation Factors[J]. Eur J Cancer,2017,83:56-70.
[2]	Zhang H L,Song Y,Yang H M,et al. Tumor Cell-intrinsic Tim-3 Promotes Lver Cancer via NF-κB/IL-6/STAT3 Axis[J]. Oncogene,2018,37:2456-2468.
[3]	Maucort-Boulch D,de Martel C,Franceschi S,et al. Fraction and Incidence of Liver Cancer Attributable to Hepatitis B and C Viruses Worldwide[J]. Int J Cancer,142:2471-2477.
[4]	Yin H S,Que R S,Liu C Y,et al. Survivin-Targeted Drug Screening Platform Identifies a Matrine Derivative WM-127 as a Potential Therapeutics Against Hepato Cellular Carcinoma[J]. Cancer Lett,2018,425:54-64.
[5]	Fraser S,Antonella V,Qiu Y Q,et al. A Prototype Therapeutic Capsule Endoscope for Ultrasound-Mediated Targeted Drug Delivery[J]. J Med Robot Res,2018,3:1-13.
[6]	Wang Y R,Yang S Y,Chen G X,et al. Barbaloin Loaded Polydopamine-Polylactide-TPGS(PLA-TPGS) Nanoparticles Against Gastric Cancer as a Targeted Drug Delivery System:Studies in Vitro and in Vivo[J]. Biochem Biophys Res Commun,2018,499(1):8-16.
[7]	Umberto C,Hsia F C,Danielle V,et al. Mesoscopic Physical Removal of Material Using Sliding Nanodiamond Contacts[J]. Sci Rep,2018,8:2994-3005.
[8]	Larsson K,Tian Y.Effect of Surface Termination on the Reactivity of Nano-sized Diamond Particle Surfaces for Bio Applications[J]. Carbon,2018,134:244-254.
[9]	Xi G F,Robinson E,Mania-Farnell B,et al. Convection-Enhanced Delivery of Nanodiamond Drug Delivery Platforms for Intracranial Tumor Treatment[J]. Nanomed-Nanotechnol Biol Med,2014,10:381-391.
[10]	Chow E K,Zhang X Q,Chen M,et al. Nanodiamond Therapeutic Delivery Agents Mediate Enhanced Chemoresistant Tumor Treatment[J]. Sci Transl Med,2011,3:73 ra 21.
[11]	Wang D X,Tong Y L,Li Y Q,et al. PEGylated Nanodiamond for Chemotherapeutic Drug Delivery[J]. Diamond Relat Mater,2013,36:26-34.
[12]	Dong Y,Cao R X,Li Y Q,et al. Folate-Conjugated Nanodiamond for Tumor-Targeted Drug Delivery[J]. RSC Adv,2015,5:82711-82716.
[13]	CAO Ruixia,TIAN Zhimei,WANG Dongxin,et al. Preparation of Folic Acid Functionalized Nanodiamonds Loading Drug and Its Targeted Research of C6 Cells[J]. J Shanxi Univ,2014,37(1):91-97(in Chinese). 曹瑞霞,田志梅,王东新,等. 叶酸功能化ND载药体系的制备及其对C6细胞靶向性研究[J]. 山西大学学报,2014,37(1):91-97.

Preparation of Lysozyme-Responsive Nanodiamond Drug-Loading System and the Effect on HepG2 Cells

RichHTML

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	GONG Tao, HUANG Yu, GUO Guoying, SU Dan, LIANG Wenting, DONG Chuan. Linear Maltodextrin Polymer Functionalized Fe₃O₄ Magnetic Nanoparticles as Drug Carriers [J]. Chinese Journal of Applied Chemistry, 2019, 36(2): 161-169.
[2]	YU Chang,SHAO Shuai,LI Hejie,LIU Guofeng,DING Binbin,MA Ping'an,LIN Jun. Upconversion Nanoparticles Decorated with Hybridization Chain Reaction DNA for Near-infrared Light Controlled Release Doxorubicin [J]. Chinese Journal of Applied Chemistry, 2018, 35(12): 1485-1491.
[3]	YU Chang, SHAO Shuai, LI Hejie, LIU Guofeng, DING Binbin, MA Ping'an, LIN Jun. Upconversion Nanoparticles Decorated with Hybridization Chain Reaction DNA for Near-infrared Light Controlled Release Doxorubicin [J]. Chinese Journal of Applied Chemistry, 2018, 35(12): 0-0.
[4]	GUO Wenyan1,2, ZHAO Minggang1*, HAO Aiyou3. Synthesis of Amphiphilic β-Cyclodextrins and Their Nanospheres in Water [J]. Chinese Journal of Applied Chemistry, 2013, 30(03): 260-264.