Abstract: It was found in this paper that only when sufficient amount of promoter molecules are adsorbed on the electrode surface,can the promoter effectively accelerate the electro-chemical reaction of cytochrome c.For a promoter such as 2,2′-bipyridine,whose molecules possess weak adsorption strength on the electrode surface,a prolonged adsorption time, such as 10 hours,is required to attain the adsorption equilibrium with sufficient amout of promoter molecules adsorbed on the electrode surface.Therefore,it is better to use a modified procedure with prolonged dipping time to minimize the differences in promoter effects brought about by variations in the adsorption strength of the promoter molecules.

Key words: bipyridine, cytochrome c, adsorption, promotion