Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (11): 1587-1594.DOI: 10.19894/j.issn.1000-0518.230147
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Han-Shui ZHANG1, Ben-Yi GONG1, Min-Lun NAN2, Yu-Fang HE3, Yu-Wei ZHAO3, Cong-Mei WU1, Yu-He YIN1()
Received:
2023-05-16
Accepted:
2023-07-25
Published:
2023-11-01
Online:
2023-12-01
Contact:
Yu-He YIN
About author:
yinyuhe@ccut.edu.cnSupported by:
CLC Number:
Han-Shui ZHANG, Ben-Yi GONG, Min-Lun NAN, Yu-Fang HE, Yu-Wei ZHAO, Cong-Mei WU, Yu-He YIN. Chemical Analysis of Sorghum Husk by UPLC-Q Exactive Plus-MS in Vitro Screening of Xanthine Oxidase Inhibitory Activity and Composition[J]. Chinese Journal of Applied Chemistry, 2023, 40(11): 1587-1594.
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URL: http://yyhx.ciac.jl.cn/EN/10.19894/j.issn.1000-0518.230147
No | tR/min | Composition | Molecular formula | Measured m/z | Fragments(MS/MS) | Delta m/z |
---|---|---|---|---|---|---|
1 | 36.90 | 2-(3-Phenylpropyl)pyridine | C14H15N | 198.128 1 | 91.054 9,106.065 7 | -0.04 |
2 | 39.64 | 2',6-Dihydroxyflavone | C | 255.065 5 | 119.029 0,211.064 9,227.070 5 | -0.02 |
3 | 39.91 | Acacetin | C16H12O5 | 285.076 5 | 242.057 6,270.052 4 | 0.67 |
4 | 42.29 | 2',3',6-Trimethoxyflavone | C18H16O5 | 313.071 4 | 280.037 8,295.060 9 | 0.09 |
5 | 44.07 | 5-Hydroxy-3'-methoxyflavone | C16H12O4 | 269.081 2 | 226.062 8,254.057 8 | -0.03 |
6 | 45.64 | 3-Hydroxy-3'-methoxyflavone | C16H12O4 | 269.081 2 | 226.063 0,254.057 8 | -0.03 |
7 | 49.87 | 2',5-Dimethoxyflavone | C17H14O4 | 283.097 0 | 239.070 7,268.073 4 | -0.03 |
8 | 57.29 | 1,5-Dihydroxyanthraquinone | C14H8O4 | 241.097 6 | 167.061 0,185.071 5,213.066 3 | -0.23 |
9 | 57.97 | Licolisoflavone A | C20H18O6 | 355.118 6 | 152.512 8 | 0.67 |
60.52 | Epigallocatechin-3-gallate | C22H18O11 | 459.144 7 | 127.035 7,181.068 2 | 0.13 | |
11 | 60.97 | Puerarin | C21H20O9 | 417.134 0 | 255.862 5 | 0.59 |
12 | 61.32 | Ipriflavone | C18H16O3 | 281.081 7 | 239.070 7 | 0.10 |
13 | 62.68 | Daidzein | C21H20O9 | 417.133 7 | 224.128 5 | 0.10 |
14 | 63.99 | Catechin gallate | C22H18O10 | 443.113 4 | 111.032 4,163.076 5,181.043 1,247.081 2 | -0.05 |
15 | 66.29 | 2,4-Dihydroxybenzophenone | C13H10O3 | 215.034 3 | 95.013 5 | 0.13 |
16 | 70.07 | 3',7-Dimethoxy-3-hydroxyflavone | C17H14O5 | 299.092 2 | 255.065 2,284.068 7 | -0.03 |
17 | 72.05 | Apigenin | C15H10O5 | 271.060 6 | 119.049 7,227.381 3,243.065 7 | -0.03 |
18 | 72.80 | Carviolin | C16H12O6 | 301.071 2 | 258.052 9,286.047 7 | -0.03 |
19 | 74.71 | Phlorizin | C21H24O10 | 437.121 0 | 166.470 1,257.116 9 | 0.04 |
77.94 | Syringetin-3-O-glucoside | C23H24O13 | 509.124 7 | 347.066 1 | -0.06 | |
78.17 | Fisetin | C15H10O6 | 287.055 5 | 149.060 2,251.012 7 | -0.03 | |
22 | 78.40 | LicochalconeA | C21H22O4 | 339.050 6 | 94.077 0 | 1.04 |
23 | 80.03 | Apigenin-7-O-glucoside | C21H20O10 | 433.129 0 | 271.053 5 | 0.58 |
24 | 80.85 | 6-Ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin | C18H16O4 | 297.076 4 | 153.018 6 | 0.09 |
25 | 82.86 | 3,7-Dihydroxy-3',4'-dimethoxyflavone | C17H14O6 | 315.086 8 | 271.060 5,300.063 6 | -0.02 |
26 | 89.26 | Isorhamnetin | C16H12O7 | 317.103 0 | 121.616 7,183.029 5,217.434 2 | 0.51 |
27 | 97.97 | Ethyl 4-(dimethylamino)benzoate | C11H15NO2 | 194.118 0 | 107.049 7,135.044 4 | -0.04 |
Table 1 QE analysis of chemical composition of sorghum husk
No | tR/min | Composition | Molecular formula | Measured m/z | Fragments(MS/MS) | Delta m/z |
---|---|---|---|---|---|---|
1 | 36.90 | 2-(3-Phenylpropyl)pyridine | C14H15N | 198.128 1 | 91.054 9,106.065 7 | -0.04 |
2 | 39.64 | 2',6-Dihydroxyflavone | C | 255.065 5 | 119.029 0,211.064 9,227.070 5 | -0.02 |
3 | 39.91 | Acacetin | C16H12O5 | 285.076 5 | 242.057 6,270.052 4 | 0.67 |
4 | 42.29 | 2',3',6-Trimethoxyflavone | C18H16O5 | 313.071 4 | 280.037 8,295.060 9 | 0.09 |
5 | 44.07 | 5-Hydroxy-3'-methoxyflavone | C16H12O4 | 269.081 2 | 226.062 8,254.057 8 | -0.03 |
6 | 45.64 | 3-Hydroxy-3'-methoxyflavone | C16H12O4 | 269.081 2 | 226.063 0,254.057 8 | -0.03 |
7 | 49.87 | 2',5-Dimethoxyflavone | C17H14O4 | 283.097 0 | 239.070 7,268.073 4 | -0.03 |
8 | 57.29 | 1,5-Dihydroxyanthraquinone | C14H8O4 | 241.097 6 | 167.061 0,185.071 5,213.066 3 | -0.23 |
9 | 57.97 | Licolisoflavone A | C20H18O6 | 355.118 6 | 152.512 8 | 0.67 |
60.52 | Epigallocatechin-3-gallate | C22H18O11 | 459.144 7 | 127.035 7,181.068 2 | 0.13 | |
11 | 60.97 | Puerarin | C21H20O9 | 417.134 0 | 255.862 5 | 0.59 |
12 | 61.32 | Ipriflavone | C18H16O3 | 281.081 7 | 239.070 7 | 0.10 |
13 | 62.68 | Daidzein | C21H20O9 | 417.133 7 | 224.128 5 | 0.10 |
14 | 63.99 | Catechin gallate | C22H18O10 | 443.113 4 | 111.032 4,163.076 5,181.043 1,247.081 2 | -0.05 |
15 | 66.29 | 2,4-Dihydroxybenzophenone | C13H10O3 | 215.034 3 | 95.013 5 | 0.13 |
16 | 70.07 | 3',7-Dimethoxy-3-hydroxyflavone | C17H14O5 | 299.092 2 | 255.065 2,284.068 7 | -0.03 |
17 | 72.05 | Apigenin | C15H10O5 | 271.060 6 | 119.049 7,227.381 3,243.065 7 | -0.03 |
18 | 72.80 | Carviolin | C16H12O6 | 301.071 2 | 258.052 9,286.047 7 | -0.03 |
19 | 74.71 | Phlorizin | C21H24O10 | 437.121 0 | 166.470 1,257.116 9 | 0.04 |
77.94 | Syringetin-3-O-glucoside | C23H24O13 | 509.124 7 | 347.066 1 | -0.06 | |
78.17 | Fisetin | C15H10O6 | 287.055 5 | 149.060 2,251.012 7 | -0.03 | |
22 | 78.40 | LicochalconeA | C21H22O4 | 339.050 6 | 94.077 0 | 1.04 |
23 | 80.03 | Apigenin-7-O-glucoside | C21H20O10 | 433.129 0 | 271.053 5 | 0.58 |
24 | 80.85 | 6-Ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin | C18H16O4 | 297.076 4 | 153.018 6 | 0.09 |
25 | 82.86 | 3,7-Dihydroxy-3',4'-dimethoxyflavone | C17H14O6 | 315.086 8 | 271.060 5,300.063 6 | -0.02 |
26 | 89.26 | Isorhamnetin | C16H12O7 | 317.103 0 | 121.616 7,183.029 5,217.434 2 | 0.51 |
27 | 97.97 | Ethyl 4-(dimethylamino)benzoate | C11H15NO2 | 194.118 0 | 107.049 7,135.044 4 | -0.04 |
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Table 2 Inhibition rate and IC50 of each sample
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