Quantitative Analysis of Cephalosporin C in Soil by High Performance Liquid Chromatography with Ultrasound-assisted Extraction and Solid-Phase Extraction

doi:10.11944/j.issn.1000-0518.2018.06.170245

Chinese Journal of Applied Chemistry ›› 2018, Vol. 35 ›› Issue (6): 722-728.DOI: 10.11944/j.issn.1000-0518.2018.06.170245

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Quantitative Analysis of Cephalosporin C in Soil by High Performance Liquid Chromatography with Ultrasound-assisted Extraction and Solid-Phase Extraction

WANG Yue^a,CAI Chen^b,LIU Huiling^b,SUN Li^c,LI Yanchao^d,MENG Zhaohong^a^*()

^aCollege of Geographic Science,Harbin Normal University,Harbin 150025,China
^bState Key Laboratory of Urban Water Resources and Environment,Harbin Institute of Technology,Harbin 150090,China
^cCollege of Geographic Science,Harbin Normal University,Harbin 150025,China
^dElectronic Engineering College,Heilongjang University,Harbin 150080,China

Received:2017-07-12 Accepted:2017-11-09 Published:2018-06-01 Online:2018-05-28
Contact: MENG Zhaohong
Supported by:
Supported by the National Natural Science Foundation of China(No.41401589), the Natural Science Foundation of Heilongjiang Province, Special Research Funds for the Universities of Heilongjiang Province(No.HDRCCX-2016Z10)

Abstract

Abstract:

A rapid and simple method based on ultrasound-assisted extraction, solid-phase extraction followed by high performance liquid chromatography(UAE-SPE-HPLC) was proposed for the determination of cephalosporin C(CPC) in soil. The optimum pre-treatment parameters for the soil samples contaminated with CPC are as follow: ultra-pure water as extraction solvent for CPC, SPE as solid phase extraction cartridge, 5.0 mL 10% methanol as rinse solvent, 2.0 mL 5% formic acid/methanol solution(volume ratio 50：50) as eluent. The results show that when 0.1% formic acid and methanol(95：5), 30 ℃ column temperature, and 254 nm detection wavelength were applied during the isocratic elution process, HPLC assay offeres a good potential for the sensitive and selective determination of CPC. The recovery rates for seven different treatments are 77.9%~98.8%, and the precision is 5.0%~6.3%(n=5). The limit of determination(LOD) and the limit of quantification(LOQ) in soil are 340.4 μg/kg and 1126.8 μg/kg, respectively. At the same time, this method was used to detect the positive soil near the pharmaceutical factory in Xinjiang. The experimental results of different batches show detected(below LOQ), 1532.1 μg/kg and detected(below LOQ), respectively. In conclusion, UAE-SPE-HPLC is simple and rapid. Its accuracy and precision are in line with the quality control requirements. It is suitable for the detection of trace CPC in soil.

Key words: cephalosporin C, soil, ultrasound-assisted extraction, solid-phase extraction, high performance liquid chromatography

WANG Yue,CAI Chen,LIU Huiling,SUN Li,LI Yanchao,MENG Zhaohong. Quantitative Analysis of Cephalosporin C in Soil by High Performance Liquid Chromatography with Ultrasound-assisted Extraction and Solid-Phase Extraction[J]. Chinese Journal of Applied Chemistry, 2018, 35(6): 722-728.

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Quantitative Analysis of Cephalosporin C in Soil by High Performance Liquid Chromatography with Ultrasound-assisted Extraction and Solid-Phase Extraction

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