Research on Simultaneous Determination of Eight Artificial Sweeteners in Food by High Performance Liquid Chromatography with Charged Aerosol Detector

doi:10.19894/j.issn.1000-0518.220267

Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (3): 436-440.DOI: 10.19894/j.issn.1000-0518.220267

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Research on Simultaneous Determination of Eight Artificial Sweeteners in Food by High Performance Liquid Chromatography with Charged Aerosol Detector

Ze-Shan LENG¹, Hong-Mei GUO², Han-Qing CAI², Jian ZHANG²^,³()

^1.Thermo Fisher Scientific，Chengdu 610011，China
^2.Sichuan Sainasi Analysis and Testing Co. ，Ltd. ，Chengdu 610023，China
^3.Analytical and Metrical Center of Sichuan Province，Chengdu 610023，China

Received:2022-08-08 Accepted:2023-01-06 Published:2023-03-01 Online:2023-03-27
Contact: Jian ZHANG
About author:1765113632@qq.com

Abstract

Abstract:

The sweeteners are separated by high performance liquid chromatography （HPLC） with 5 mmol/L ammonium formate-acetonitrile gradient elution and quantified by charged aerosol detector （CAD） to establish a method for the simultaneous determination of eight artificial sweeteners in food by HPLC-CAD. The linearity of acesulfame， sodium saccharin， cyclamate， sucralose， aspartame， alitame， advantame and neotame in the range is good， and the correlation coefficient is above 0.999. The limit of detection and quantitation is 5～30 μg/L and 20～150 μg/L， respectively. The recovery rates are 88.5% to 102.8%， and the relative standard deviations are less than 2.16%. The method is simple， rapid， sensitive and reproducible， and can be used for the detection of 8 sweeteners in food.

Key words: Artificial sweeteners, High performance liquid chromatography, Charged aerosol detector

CLC Number:

O657.7

Ze-Shan LENG, Hong-Mei GUO, Han-Qing CAI, Jian ZHANG. Research on Simultaneous Determination of Eight Artificial Sweeteners in Food by High Performance Liquid Chromatography with Charged Aerosol Detector[J]. Chinese Journal of Applied Chemistry, 2023, 40(3): 436-440.

Figures/Tables 4

References 11

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Sweetener	Linear range/（mg·L^-1）	Linear equation	R²	LOD/（μg·L^-1）	LOQ/（mg·L^-1）	LOQ of national standard/（mg·L^-1）
Acesulfame	0.15～10	y=0.0203x+0.0009	0.999 1	30	0.15	4
Sodium saccharin	0.12～10	y=0.0371x+0.0017	0.999 9	25	0.12	1
Cyclamate	0.15～10	y=0.0343x-0.0008	0.999 8	30	0.15	10
Sucralose	0.05～10	y=0.0765x+0.0035	0.999 5	15	0.05	20
Aspartame	0.02～5	y=0.1824x+0.0068	0.999 2	5	0.02	5
Alitame	0.02～2.5	y=0.1892x+0.0051	0.999 5	5	0.02	5
Advantame	0.02～5	y=0.1537x+0.0032	0.999 1	5	0.02	0.05
Neotame	0.02～2.5	y=0.1791x+0.0046	0.999 3	5	0.02	0.2

Sweetener	Linear range/（mg·L^-1）	Linear equation	R²	LOD/（μg·L^-1）	LOQ/（mg·L^-1）	LOQ of national standard/（mg·L^-1）
Acesulfame	0.15～10	y=0.0203x+0.0009	0.999 1	30	0.15	4
Sodium saccharin	0.12～10	y=0.0371x+0.0017	0.999 9	25	0.12	1
Cyclamate	0.15～10	y=0.0343x-0.0008	0.999 8	30	0.15	10
Sucralose	0.05～10	y=0.0765x+0.0035	0.999 5	15	0.05	20
Aspartame	0.02～5	y=0.1824x+0.0068	0.999 2	5	0.02	5
Alitame	0.02～2.5	y=0.1892x+0.0051	0.999 5	5	0.02	5
Advantame	0.02～5	y=0.1537x+0.0032	0.999 1	5	0.02	0.05
Neotame	0.02～2.5	y=0.1791x+0.0046	0.999 3	5	0.02	0.2

Sweetener	Additive amount/（mg·L^－1）	Recovery rate/%	RSD/%	Sweetener	Additive amount/（mg·L^－1）	Recovery rate/%	RSD/%
Acesulfame	0.25	88.5～93.6	2.16	Aspartame	0.05	93.2～97.6	1.84
	1.0	92.6～96.3	1.32		0.5	96.3～98.8	0.98
	5.0	96.5～98.3	1.08		2.5	97.1～98.6	0.93
Sodium saccharin	0.25	89.6～93.4	1.85	Alitame	0.05	92.8～96.7	1.59
	1.0	96.1～99.8	1.26		0.25	93.6～95.8	1.31
	5.0	96.7～99.2	1.03		1.5	97.9～99.1	1.17
Cyclamate	0.25	88.7～92.8	1.84	Advantame	0.05	94.2～101.3	2.06
	1.0	95.5～99.4	1.14		0.5	98.2～102.7	1.64
	5.0	96.5～98.8	1.12		2.5	96.5～99.4	1.22
Sucralose	0.1	90.6～94.7	1.58	Neotame	0.05	95.9～102.8	2.13
	1.0	98.6～101.2	0.99		0.25	96.7～100.5	1.27
	5.0	98.3～100.1	0.85		1.5	97.4～100.2	1.05

Sweetener	Additive amount/（mg·L^－1）	Recovery rate/%	RSD/%	Sweetener	Additive amount/（mg·L^－1）	Recovery rate/%	RSD/%
Acesulfame	0.25	88.5～93.6	2.16	Aspartame	0.05	93.2～97.6	1.84
	1.0	92.6～96.3	1.32		0.5	96.3～98.8	0.98
	5.0	96.5～98.3	1.08		2.5	97.1～98.6	0.93
Sodium saccharin	0.25	89.6～93.4	1.85	Alitame	0.05	92.8～96.7	1.59
	1.0	96.1～99.8	1.26		0.25	93.6～95.8	1.31
	5.0	96.7～99.2	1.03		1.5	97.9～99.1	1.17
Cyclamate	0.25	88.7～92.8	1.84	Advantame	0.05	94.2～101.3	2.06
	1.0	95.5～99.4	1.14		0.5	98.2～102.7	1.64
	5.0	96.5～98.8	1.12		2.5	96.5～99.4	1.22
Sucralose	0.1	90.6～94.7	1.58	Neotame	0.05	95.9～102.8	2.13
	1.0	98.6～101.2	0.99		0.25	96.7～100.5	1.27
	5.0	98.3～100.1	0.85		1.5	97.4～100.2	1.05

Sample	Detection times of each sweetener
Sample	Acesulfame	Sodium saccharin	Cyclamate	Sucralose	Aspartame	Alitame	Advantame	Neotame
Cake（15 kinds）	3	2	12	0	0	0	0	0
Bread （15 kinds）	3	4	11	1	0	0	0	0
Beverage （10 kinds）	0	1	0	1	9	1	0	10
Liquor （10 kinds）	0	0	0	2	0	0	0	0

Research on Simultaneous Determination of Eight Artificial Sweeteners in Food by High Performance Liquid Chromatography with Charged Aerosol Detector

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