Synthesis and Spectroscopic Properties of a Cryptate Rare Earth Fluorescence Complex

doi:10.11944/j.issn.1000-0518.2016.03.160232

Chinese Journal of Applied Chemistry ›› 2017, Vol. 34 ›› Issue (3): 361-366.DOI: 10.11944/j.issn.1000-0518.2016.03.160232

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Synthesis and Spectroscopic Properties of a Cryptate Rare Earth Fluorescence Complex

CHANG Yu,XIE Wenbing,ZHOU Shihong,LI Junling,LIU Baofeng,PAN Lihua()

Laboratory of Polymer Composites Engineering, Changchun Institute of Applied Chemistry Chinese Academy of Sciences,Changchun 130022,China

Received:2016-06-02 Accepted:2016-08-25 Published:2017-02-27 Online:2017-02-27
Contact: PAN Lihua
Supported by:
Supported by the Science and Technology Development Project of Jilin Province of China(No.20150204010YY)

Abstract

Abstract:

A bifuntional cryptate chelate 2,6-{N,N',N,N'-[bis(2,2'-bipyridine-6,6'-dimethyl)]bis(aminomethyl)}-pyridine-3,5-diethyl diester for homogeneous time-resolved fluorimmunoassay product was synthesized from the raw material diethyl 2,6-dimethylpyridine-3,5-dicarboxylate through N-bromobutanimide(NBS) bromination, electrophilic substitution. The structure and property were confirmed by differential thermal analysis(DTA) Fourier transform infrared spectroscopy(FTIR), proton nuclear magnetic resonance spectroscopy(¹H NMR) and mass spectrometry(MS). Also, the spectral properties of the Eu(Ⅲ)-cryptate chelate, such as absorption spectra, emission spectra, and fluorescence lifetime, were discussed. The fluorescence spectroscopic properties of Eu(Ⅲ)-cryptate are as follows: the excitation peak is at 322 nm, the narrow emission spectrum peaks are at 597 nm(⁵D₀-⁷F₁) and 618 nm(⁵D₀-⁷F₂), respectively, the Stokes shift is 290 nm, the fluorescence lifetime is 918 μs, and the fluorescence quantum yield is Φ=0.249.

Key words: homogeneous time-resolved fluorescence immunoassay, chelate, synthesis, cryptate bipyridine derivative

CHANG Yu, XIE Wenbing, ZHOU Shihong, LI Junling, LIU Baofeng, PAN Lihua. Synthesis and Spectroscopic Properties of a Cryptate Rare Earth Fluorescence Complex[J]. Chinese Journal of Applied Chemistry, 2017, 34(3): 361-366.

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Synthesis and Spectroscopic Properties of a Cryptate Rare Earth Fluorescence Complex

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