Abstract: A novel imprinted composite film electrochemical sensor was fabricated based on molecularly imprinted composite film for the determination of chloramphenicol(CAP). The imprinted composite film was prepared by electrochemical polymerization of pyrrole(PPY) and polyaniline(PAN) on the surface of stainless steel needle(SSN) electrode in the presence of CAP as a template. The morphology and electrochemical property of the film were characterized by SEM and differential pulse voltammetry with ferricyanide as an electrochemical probe, respectively. The film exhibited the good sensitivity, selectivity and the ability of regeneration. A linear relationship between the current of electrochemical probe and the concentration of CAP was found in the range of 5.00×10-8～1.05×10-6 mol/L with a detection limit of 2.09×10-9 mol/L.

Key words: molecular , impinted film, electrochemical polymerization, electrochemical detection, chloramphenicol, aniline, pyrrole