Abstract:

Inhibiting effect of the curcumin(Cur) in combination with cis-diamminedichloroplatinum（DDP） on proliferation of COC1 cells was analyzed with MTT colorimetric assay. The morphological changes of COC1 cells were observed by inverted microscope. The result indicated that DDP of 2.5～10 mg/L dose combined with curcumin(Cur) of 5 mg/L dose could enhance the inhibiting effect and induce apoptosis compared with the case when only DDP was used. Meanwhile, curcumin of 5 mg/L dose could improve the sensitization of COC1 cells to DDP with 2.66 times. Optical microscopy results showed that COC1 cells had a marked decreased proliferation and a great amount of cells necrosis in DDP+Cur group and DDP group than the control group. COC1 cells had lower diopter, scatter array, serious vacuolization treated with DDP+Cur. The HPLC result indicated that the contents of DDP within tumor cells decreased lightly after dosage of Cur, but it had no statistically significant(P＞0.05). In summary, curcumin combine with DDP can enhance the inhibiting effect on proliferation of COC1 cells, meanwhile, curcumin can improve the sensitization of COC1 cells to chemotherapeutics. However, the curcumin is unlikely to affect the amount of DDP within cells.

Key words: Curcumin, cis-Diamminedichloroplatinum, Inhibiting effect, MTT, HPLC