Abstract:

A high performance liquid chromatographic method was developed for simultaneous determining the contents of galanthamine and lycorine in Lycoris Herb. The chromatographic analysis was carried out on a phenomenex-C18 column. The mobile phase was acetonitrile(A)-0.1%trifluoroacetic(TFA)(B) with gradient elution. The detection wavelength was set at 289 nm. The calibration curves for galanthamine and lycorine showed good linearity in the range of 0.5～10 mg/L(r=0.9999). The detection limits were 0.09 mg/L for galanthamine and 0.15 mg/L for lycorine at the signal-to-noise ratio of 3, and the average recoveries were 99.53% and 96.82%, respectively. The method is simple, rapid, accurate and suitable for the determination of galanthamine and lycorine in Lycoris Herb.

Key words: HPLC, Lycoris Herb, galanthamine, lycorine