Abstract:

ABSTRACT Carbonic anhydrase(CA) was immobilized onto the surface of hollow fiber membrane of polymethyl pentene(PMP) without any active functional groups by using sulfosuccinimidyl [perfluoroazidobenzamido] ethyl-1,3-dithiopropionate (SFAD), a photoactivatable heterobifunctional crosslinker. Using p-nitrophenyl acetate (p-NPA) as a substrate, the activity, concentration, storage stability and re-usability of immobilized CA on PMP hollow fibers were studied by ultraviolet spectrophotometer. The preliminary data showed that CA could be successfully introduced onto PMP surface with catalysis activity and immobilization efficiency.The activity of covalently immobilized CA increased with the increase of concentration of SFAD, and the maximum was 85.86% of the theoretical activity of CA spread on PMP surface in monolayer in studied range. Covalently immobilized CA showed higher reusability compared to physically adsorbed CA, and higher storage stability compared to free CA in solution at 37℃.

Key words: Polymethyl pentene, Photoreactive crosslinker, Carbonic anhydrase, p-nitrophenyl acetate