Synthesis of α-Glycosyl-Stevia Sugar Catalyzed by Glycosyltransferase(Ⅱ)

Chinese Journal of Applied Chemistry ›› 1996, Vol. 0 ›› Issue (6): 24-27.

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Synthesis of α-Glycosyl-Stevia Sugar Catalyzed by Glycosyltransferase(Ⅱ)

Shi Zuoqing¹, Zhu Boru¹, Shi Rongfu¹, Li Xiaoting¹, He Binglin¹, Jia Zhifen², Qiao Jinsheng²

1. Institute of Polymer Chemistry, Nankai University, The State Key Laboratory of Functional Polymer Materials for Ad, Tianjin 300071;
2. Hecheng High Science and Technology Developing Cmnpany, Nankai University, Tianjin

Received:1996-03-30 Revised:1996-08-07 Published:1996-12-10 Online:1996-12-10

Abstract

Abstract: Anion cellulosic beads were used as support for immobilization of glycosyltransferase GT-1.The immobilized enzyme was used to catalyze the synthesis of α-glycosylstevia sugar.Various influencing factors were discussed.The result showed that when the reaction reached to a certain extent,transglycosylated amount changed very little with increase of amounts of enzyme and starch.The activity of immobilized GT-1 lost very little after repeated uses for 8 times.

Key words: stevia sugar, immobilization, glycosyltransferase, enzyme-catalyzed synthesis

Shi Zuoqing, Zhu Boru, Shi Rongfu, Li Xiaoting, He Binglin, Jia Zhifen, Qiao Jinsheng. Synthesis of α-Glycosyl-Stevia Sugar Catalyzed by Glycosyltransferase(Ⅱ)[J]. Chinese Journal of Applied Chemistry, 1996, 0(6): 24-27.

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Synthesis of α-Glycosyl-Stevia Sugar Catalyzed by Glycosyltransferase(Ⅱ)

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