Chinese Journal of Applied Chemistry ›› 1993, Vol. 0 ›› Issue (3): 16-19.

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STUDY ON THE KINETICS OF IMMOBILIZED ENZYME REACTION WITH INTERNAL DIFFUSION LIMITATION USING SINGLE SIZE PARTICLE

Zou Wenqiao1, Feng Yangjie1, Li Bing2   

  1. 1. East China Institute of Chemical Technology, 200237 Shanghai;
    2. Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai
  • Received:1992-05-03 Revised:1992-12-08 Published:1993-06-10 Online:1993-06-10

Abstract: A method was suggested for the study of immobilized enzyme kinetics with internal diffusion limitations in a fixed-bed reactor using single size immobilized enzyme. According to the characters of the reactions at enough high and low concentrations of substrates, corresponding to zero and first order reactions respectively, the intrinsic kinetics parameters and internal diffusion parameters for reaction of glucose isomerization were determined. The values of effectiveness factor were calculated and compared with that obtained in the experiment.

Key words: immobilized enzyme, reaction kinetics