Abstract:

A screened lipase from Candida sp.GXU08 strain was used to catalyze the synthesis reaction of cyclopentadecanolide from methyl 15-hydroxypentadecanate. The lipase solution was dispersed in cyclohexane by ultrasonication to form an aqueous/organic solvent emulsion system. Different effects of reaction conditions on the production of cyclopentadecanolide were studied. The maximal production of cyclopentadecanolide was 47.77×10-3 mg/U in the emulsion system under the reaction conditions:40 ℃, 180 r/min, 1 g pH=7.0 lipase solution, 8 mmol/L substrate, 72 h. The production in the emulsion system was 3.285 times of that in cyclohexane organic system. It was also found that reusing the lipase once would greatly increase the production of cyclopentadecanolide. So the lipase powder could be replaced by lipase solution to catalyze the synthesis of cyclopentadecanolide, which could enhance the conversion of the reaction, save time and reduce the use of energy.

Key words: emulsion system, ultrasonic, lipase, cyclopentadecanolide