Immobilized-Lipase(Candida sp.99-125)-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems

doi:10.3724/SP.J.1095.2013.20496

Chinese Journal of Applied Chemistry

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Immobilized-Lipase(Candida sp.99-125)-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems

ZHONG Hui¹, FANG Zheng¹, ZOU Baohua¹, LI Xin², GUO Kai^2*

(1.School of Pharmaceutical Sciences;2.College of Biotechnology and Pharmaceutical Engineering,
Nanjing University of Technology,Nanjing 211816,China)

Received:2012-10-31 Revised:2012-12-18 Published:2013-09-10 Online:2013-09-10
Contact: guo

Abstract

Abstract: The esterification between oleic acid and alkyl alcohols in solvent-free systems were catalyzed by an immobilized lipase from Candida sp.99-125. The effects of several factors including enzyme concentration, temperature, molar ratio of oleic acid to alkyl alcohols, and structure of alcohols have been also investigated. The results indicated that the reactions catalyzed by lipase at 20 ℃ with the presence of 3%(mass fraction) lipase, a 1∶1 molar ratio of oleic acid to alcohols, afforded products in high yields. Methanol has certain toxicity on the activity of the lipase. The enzyme showed high selectivity to primary alcohols and low selectivity to secondary and tertiary alcohols because of the sterical effect. It showed high activity to long chain fatty acids and low activity to polybasic acids and polyhydric alcohols with branched chain. The lipase showed no appreciable loss in activity after being continuously operated for 5 times. The enzymatic synthesis gave purer products, compared with the conventional chemical system.

Key words: enzymatic catalysis, immobilized lipase, esterification, oleates, solvent-free system

CLC Number:

O629.8

ZHONG Hui1, FANG Zheng1, ZOU Baohua1, LI Xin2, GUO Kai2*. Immobilized-Lipase(Candida sp.99-125)-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2013.20496.

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Immobilized-Lipase(Candida sp.99-125)-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems

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