Effective Application of Dihydromyricetin in Vine Tea Extract and Its Skin Care Efficacy

doi:10.19894/j.issn.1000-0518.250156

Chinese Journal of Applied Chemistry ›› 2026, Vol. 43 ›› Issue (2): 235-248.DOI: 10.19894/j.issn.1000-0518.250156

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Effective Application of Dihydromyricetin in Vine Tea Extract and Its Skin Care Efficacy

Yue TAN¹, Zhi-Cheng YE¹, Na ZHAO², Qi-Miao SHAO², Ya-Zhuo SHANG¹()

^1.Laboratory of Cosmetics，School of Chemistry & Molecular Engineering，East China University of Science and Technology，Shanghai 200237，China
^2.Yucca Elements （Hangzhou） Technology Co. ，Ltd. ，Hangzhou 310018，China

Received:2025-04-14 Accepted:2025-10-10 Published:2026-02-01 Online:2026-03-06
Contact: Ya-Zhuo SHANG
About author:shangyazhuo@ecust.edu.cn
Supported by:
the National Natural Science Foundation of China(22308094)

Abstract

Abstract:

Dihydromyricetin， the main active substance in vine tea extract， exhibits anti-inflammatory and antioxidant properties， making it highly promising for applications in functional cosmetics. However， its low water solubility and precipitation tendency have limited its practical applilation in cosmetic formulations. To address these challenges， this study developed a supramolecular deep eutectic solvent system incorporating dihydromyricetin from vine tea extract， using synergistic components such as betaine and glycerol. The system was designed to enhance the solubility and stability of dihydromyricetin， thereby overcoming its application limitations. In this paper， the formation and stabilization mechanism of the prepared supramolecular system were investigated using experiments combined with theoretical calculations. Results from Fourier-transform infrared spectroscopy （FT-IR） and theoretical calculations revealed that the system's stability is primarily attributed to strong non-covalent interactions among the components. The results of transdermal and cytological tests confirmed that the vine tea supramolecular system has good transdermal properties and anti-inflammatory efficacy. The results of clinical efficacy tests showed that the product containing vine tea supramolecular system （mass fraction 0.5%） could significantly improve the skin erythema and wrinkles of the participants. This research not only provides a reliable approach for the effective application of dihydromyricetin in vine tea extract， but also lays a foundation for the development of high-performance functional skincare products.

Key words: Dihydromyricetin, Supramolecular, Transdermal properties, Anti-inflammatory, Anti-wrinkle

CLC Number:

O629

Yue TAN, Zhi-Cheng YE, Na ZHAO, Qi-Miao SHAO, Ya-Zhuo SHANG. Effective Application of Dihydromyricetin in Vine Tea Extract and Its Skin Care Efficacy[J]. Chinese Journal of Applied Chemistry, 2026, 43(2): 235-248.

Figures/Tables 15

Fig.1 Photos of aqueous solutions of different mass fractions of supramolecular vine tea system

Fig.2 FT-IR spectra of supramolecular vine tea system， betaine/glycerol/dipropylene glycol system and their components

Fig.3 Microscopic morphology of supramolecular vine tea system （mass fraction 0.5%） self-assembled body in aqueous environment

Fig.4 （A） Molecular surface electrostatic potential diagram；（B） Interaction region indicator contour map of supramolecular vine tea system

Fig.5 IRI contour plots of supramolecular vine tea system in an aqueous environment

Fig.6 （A） Liquid chromatograms of dihydromyricetin standard solution （0.2 mg/mL）；（B） Standard curve of dihydromyricetin；（C） Liquid chromatograms of supramolecular vine tea system solution （mass fraction 0.1%）；（D） Liquid chromatograms of vine tea extract solution （0.2 mg/mL）

Fig.7 Transdermal permeation curve of dihydromyricetin in different systems

Fig.8 Effect of supramolecular vine tea system on the viability of RAW264.7 cells

Fig.9 Effect of supramolecular vine tea system on the release of NO in LPS-induced RAW264.7 cells

Fig.10 Effect of supramolecular vine tea system on the contents of IL-1β in LPS-induced RAW264.7 cells

Fig.11 Changes in the value of the skin redness index a* in participants

Fig.12 Typical case of text group participant's facial red zone changes

Fig.13 Changes in （A） under-eye wrinkle area，（B） frown line area，（C） under-eye wrinkle volume and （D） frown line volume in participants

Fig.14 Typical cases of wrinkle changes in the text group participant's under-eye wrinkles （top） and frown lines （bottom）（VISIA images）

Fig.15 Typical cases of wrinkle changes in the text group participant's under-eye wrinkles （top） and frown lines （bottom）（Antera 3D images）

References 32

[1]	张友胜，杨伟丽，熊皓平. 显齿蛇葡萄基本成分研究［J］. 天然产物研究与开发， 2001（5）： 46-48.
	ZHANG Y S， YANG W L， XIONG H P. Basic constituent of Ampelopsis grossedentata［J］. Nat Prod Res Dev， 2001（5）： 46-48.
[2]	张友胜，宋松泉，刘军. 藤茶的典籍［J］. 生命世界， 2021（5）： 24-27.
	ZHANG Y S， SONG S Q， LIU J. The canon of vine tea［J］. Life World， 2021（5）： 24-27.
[3]	张桐，丁桂清，乔曦，等. 藤茶及其活性成分二氢杨梅素的药理作用［J］. 辽宁中医药大学学报， 2024， 26（7）： 107-113.
	ZHANG T， DING G Q， QIAO X， et al. Pharmacological effects of vine tea and its active component dihydromyricetin［J］. J Liaoning Univ Tradit Chin Med， 2024， 26（7）： 107-113.
[4]	何桂霞，裴刚，李斌，等. 二氢杨梅素的稳定性研究［J］. 中国新药杂志， 2007（22）： 1888-1890.
	HE G X， PEI G， LI B， et al. Studies on stability of dihydromyricetin［J］. Chin J New Drugs， 2007（22）： 1888-1890.
[5]	耿艳艳，郭爱秀，金苏英，等. 藤茶的功效及其应用研究进展［J］. 饮料工业， 2019， 22（1）： 71-74.
	GENG Y Y， GUO A X， JIN S Y， et al. Research progress in effect and use of vine tea［J］. Beverage Ind， 2019， 22（1）： 71-74.
[6]	ACHKAR E T， GREIGE-GERGES H. Fourmentin S. basics and properties of deep eutectic solvents： a review［J］. Environ Chem Lett， 2021， 19（4）： 3397-3408.
[7]	PRABHUNE A， DEY R. Green and sustainable solvents of the future： deep eutectic solvents［J］. J Mol Liq， 2023， 379（11）： 121676.
[8]	RUß C， KÖNIG B. Low melting mixtures in organic synthesis-an alternative to ionic liquids？［J］. Green Chem， 2012， 14（11）： 2969-2982.
[9]	ABBOTT A P， CAPPER G， DAVIES D L， et al. Novel solvent properties of choline chloride/urea mixtures［J］. Chem Commun， 2003（1）： 70-71.
[10]	ZHANG M， ZHANG X， LIU Y， et al. Insights into the relationships between physicochemical properties， solvent performance， and applications of deep eutectic solvents［J］. Environ Sci Pollut Res， 2021， 28（27）： 35537-35563.
[11]	SOLTANMOHAMMADI F， JOUYBAN A， SHAYANFAR A. New aspects of deep eutectic solvents： extraction， pharmaceutical applications， as catalyst and gas capture［J］. Chem Pap， 2021， 75（2）： 439-453.
[12]	XIE B， JIANG Q， ZHU F， et al. Transdermal delivery of amphotericin B using deep eutectic solvents for antifungal therapy［J］. Chin Chem Lett， 2025， 36（5）： 110508.
[13]	LU B， LIU T， WANG H， et al. Ionic liquid transdermal delivery system： progress， prospects， and challenges［J］. J Mol Liq， 2022， 351（7）： 118643.
[14]	BAI D， WANG Z， XIAO Y， et al. Transdermal delivery of elastin peptide assisted by betaine-based deep eutectic solvent to ameliorate skin photoaging［J］. Biomater Adv， 2024， 163（8）： 213965.
[15]	STEPHENS P J， DEVLIN F J， CHABALOWSKI C F， et al. Ab initio calculation of vibrational absorption and circular dichroism spectra using density functional force fields［J］. J Phys Chem， 1994， 98（45）： 11623-11627.
[16]	WEIGEND F， AHLRICHS R. Balanced basis sets of split valence， triple zeta valence and quadruple zeta valence quality for H to Rn： design and assessment of accuracy［J］. Phys Chem Chem Phys， 2005， 7（18）： 3297-3305.
[17]	LU T， CHEN F. Multiwfn： a multifunctional wavefunction analyzer［J］. J Comput Chem， 2012， 33 （5）： 580-592.
[18]	HUMPHREY W， DALKE A， SCHULTEN K. VMD： visual molecular dynamics［J］. J Mol Graphics， 1996， 14（1）： 33-38.
[19]	柳亚锋，刘岱拯，谢珍茗. 6种常用防晒剂的透皮吸收和安全评估［J］. 日用化学工业， 2021， 51（11）： 1088-1094.
	LIU Y F， LIU D Z， XIE Z M. Transdermal absorption and safety assessment of six commonly used sun-screening agents［J］. China Surfactant Deterg Cosmet， 2021， 51（11）： 1088-1094.
[20]	夏琳，邱桂学. 化学科学的研究新领域——超分子化学［J］. 化学推进剂与高分子材料， 2007（1）： 33-37.
	XIA L， QIU G X. New field of chemistry science research-supramolecular chemistry［J］. Chem Propellants Polym Mater， 2007（1）： 33-37.
[21]	MACCHIONI V， CARBONE K， CATALDO A， et al. Lactic acid-based deep natural eutectic solvents for the extraction of bioactive metabolites of humulus lupulus L.： supramolecular organization， phytochemical profiling and biological activity［J］. Sep Purif Technol， 2021， 264（11）： 118039.
[22]	ALAVI S M， YEGANEGI S. DFT study of structures and hydrogen bonds of imidazolium based halogen-free boron containing dicationic ionic liquids［J］. J Mol Liq， 2018， 256（8）： 330-343.
[23]	LEE H J， HYUN E A， YOON W J， et al. In vitro anti-inflammatory and anti-oxidative effects of cinnamomum camphora extracts［J］. J Ethnopharmacol， 2006， 103（2）： 208-216.
[24]	KIM H K， CHEON B S， KIM Y H， et al. Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure-activity relationships［J］. Biochem Pharmacol， 1999， 58（5）： 759-765.
[25]	HEO S J， YOON W J， KIM K N， et al. Evaluation of anti-inflammatory effect of fucoxanthin isolated from brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages［J］. Food Chem Toxicol， 2010， 48（8）： 2045-2051.
[26]	BENT R， MOLL L， GRABBE S， et al. Interleukin-1 beta-a friend or foe in malignancies？［J］. Int J Mol Sci， 2018， 19（8）： 2155.
[27]	EIGLER A， SINHA B， HARTMANN G， et al. Taming TNF： strategies to restrain this proinflammatory cytokine［J］. Immunol Today， 1997， 18（10）： 487-492.
[28]	ZHOU J， OTTEWELL P D. The role of IL-1B in breast cancer bone metastasis［J］. J Bone Oncology， 2024， 46（3）： 100608.
[29]	赵小敏，赵云珊，瞿欣. 图像分析法在化妆品功效评价中的应用［J］. 日用化学品科学， 2016， 39（1）： 29-33.
	ZHAO X M， ZHAO Y S， QU X. Application of quantitative image analysis on clinical efficacy evaluation of cosmetics［J］. Deterg Cosmet， 2016， 39（1）： 29-33.
[30]	XU D T， YAN J N， CUI Y， et al. Quantifying facial skin erythema more precisely by analyzing color channels of the VISIA red images［J］. J Cosmet Laser Ther， 2016， 18（5）： 296-300.
[31]	左盼盼，柯燕子，朱芷汀，等. 基于皮肤颜色分析的化妆品人体舒缓功效评价方法比较［J］. 中国洗涤用品工业， 2024（4）： 100-105.
	ZUO P P， KE Y Z， ZHU Z T， et al. Comparison of evaluation methods of human soothing efficacy of cosmetics based on skin color analysis［J］. China Clean Ind， 2024（4）： 100-105.
[32]	李诚桐，赵华，王敏. 化妆品功效评价（Ⅸ）——图像分析法在化妆品功效评价中的应用［J］. 日用化学工业， 2018， 48（10）： 551-557.
	LI C T， ZHAO H， WANG M. Efficacy evaluation of cosmetics（Ⅸ） application of image analysis method in the evaluation of cosmetic efficacy［J］. China Surfactant Deterg Cosmet， 2018， 48（10）： 551-557.

Effective Application of Dihydromyricetin in Vine Tea Extract and Its Skin Care Efficacy

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