Preparation of Graphene Nano Drug-Loading System and Its Killing Effect on Human Oral Squamous Cell Carcinoma Cells

doi:10.11944/j.issn.1000-0518.2019.05.180351

Chinese Journal of Applied Chemistry ›› 2019, Vol. 36 ›› Issue (5): 564-570.DOI: 10.11944/j.issn.1000-0518.2019.05.180351

• Full Papers • Previous Articles Next Articles

Preparation of Graphene Nano Drug-Loading System and Its Killing Effect on Human Oral Squamous Cell Carcinoma Cells

ZHANG Yajing^a,JI Peng^a,HAN Deming^a,ZHAO Lihui^a^*(),XU Yajuan^b^*(),LIU Wenshu^c

^aCollege of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022,China
^bJilin Cancer Hospital,Changchun 130012,China
^cThe First Hospital of Jilin University,Changchun 130021,China

Received:2018-11-06 Accepted:2019-02-28 Published:2019-05-01 Online:2019-05-06
Contact: ZHAO Lihui,XU Yajuan
Supported by:
Supported by the Jilin Provincial Science and Technology Agen(No.JJKH20170604KJ)

Abstract

Abstract:

Graphene oxide was synthesized by chemical redox method. The graphene oxide nanoparticles were prepared by ultrasonic fragmentation and peeling, and functionalized by polyethylene glycol(PEG). The modified graphene oxide was loaded with cisplatin. The graphene nano-drug delivery system was characterized by scanning electron microscopy(SEM), ultraviolet-visible absorption spectroscopy(UV-Vis) and Fourier transform infrared spectroscopy(FTIR). The killing effect of graphene nano-drug delivery system on oral squamous cell carcinoma human oral squamous cell carcinoma(KB) cells was examined by cell survival rate experiment(MTT) method. The results demonstrate that the loading rate of graphene nano-drug-loading system is 42.4%. Polyethylene glycol modified nano graphene oxide can reduce the cytotoxicity of nano graphene oxide and improve its biocompatibility. It has dual killing effect on KB cells, which provides a theoretical basis for the clinical application of nano graphene oxide in cancer treatment.

Key words: nano graphene oxide, cisplatin, cell survival rate experiment, human oral squamous cell carcinoma cells, cytotoxicity

ZHANG Yajing,JI Peng,HAN Deming,ZHAO Lihui,XU Yajuan,LIU Wenshu. Preparation of Graphene Nano Drug-Loading System and Its Killing Effect on Human Oral Squamous Cell Carcinoma Cells[J]. Chinese Journal of Applied Chemistry, 2019, 36(5): 564-570.

References

[1]	Warnakulasuriya S.Global Epidemiology of Oral and Oropharyngeal Cancer[J]. Oral Oncol,2009,45(4):309-316.
[2]	LUO Yi.Diagnosis and Treatment Strategies of Locally Advanced/Distant Metastasis of Head and Neck Squamous Cell Carcinomas[J]. Anti-tumor Pharm,2016,6(1):1-3(in Chinese). 罗以. 局部晚期/远处转移的头颈部鳞癌的诊治策略[J]. 肿瘤药学,2016,6(1):1-3.
[3]	Yin Q,Chen W.Research Progress of Pathogeny and Treatment of Oral Cancer[J]. Cancer Res Clin,2014,26(8):573-576.
[4]	Nagler R M.Molecular Aspects of Oral Cancer[J]. Anticancer Res,2002,22(5):2977-2980.
[5]	Singh D P,Herrera C E,Singh B,et al. Graphene Oxide:An Efficient Material and Recent Approach for Biotechnological and Biomedical Applications[J]. Mater Sci Eng C,2018,86(3):173-197.
[6]	Banerjee A N.Graphene and Its Derivatives as Biomedical Materials:Future Prospects and Challenges[J]. Interface Focus A Theme Supplement J R Soc Interface,2018,8(3):1-22.
[7]	Bramini M,Alberini G,Colombo E,et al. Interfacing Graphene-Based Materials with Neural Cells[J]. Front Syst Neurosci,2018,12(1):1-22.
[8]	Lee Y,Seo T H,Lee S,et al. Neuronal Differentiation of Human Mesenchymal Stem Cells in Response to the Domain Size of Graphene Substrates[J]. J Biomed Mater Res Part A,2018,106(1):43-51.
[9]	Mohammadrezaei D,Golzar H,Rezai R M,et al. In vitro Effect of Graphene Structures as an Osteoinductive Factor in Bone Tissue Engineering:A Systematic Review[J]. J Biomed Mater Res Part A,2018,106(8):2284-2343.
[10]	Fisher C,Rider A E,Han Z J,et al. Applications and Nanotoxicity of Carbon Nanotubes and Graphene in Biomedicine[J]. J Nanomater,2012,2012(8):1-19.
[11]	JIAO Fang,ZHOU Guoqiang,CHEN Chunying.Trends in Studies on Chemical Modification and Biomedical Applications of Fullerenes[J]. J Ecotoxicol,2010,5(4):469-480(in Chinese). 焦芳,周国强,陈春英. 富勒烯化学修饰与生物医学应用研究进展[J]. 生态毒理学报,2010,5(4):469-480.
[12]	BAI Ru,WANG Wen,JIN Xinglong,et al. Review on Biological Security of Nanomaterials[J]. J Environ Health,2007,24(1):59-61(in Chinese). 白茹,王雯,金星龙,等. 纳米材料生物安全性研究进展[J]. 环境与健康杂志,2007,24(1):59-61.
[13]	Akduman C,Özgüney I,Kumbasar E P A. Preparation and Characterization of Naproxen-Loaded Electrospun Thermoplastic Polyurethane Nanofibers as a Drug Delivery System[J]. Mater Sci Eng C,2016,64(5):383-390.
[14]	Li W D,Sun X Y.Mechanisms by Which Tumor Hypoxic and Acidic Microenvironments Affect Immunotherapy[J]. World Chinese J Digestol,2017,25(21):1934-1944.
[15]	Zhao F,Zhao Y,Liu Y,et al. Cellular Uptake, Intracellular Trafficking, and Cytotoxicity of Nanomaterials[J]. Small,2011,7(10):1322-1337.
[16]	Seabra A B,Paula A J,Lima R D,et al. Nanotoxicity of Graphene and Graphene Oxide[J]. Chem Res Toxicol,2014,27(2):159-168.
[17]	Tian L,Pei X,Zeng Y,et al. Functionalized Nanoscale Graphene Oxide for High Efficient Drug Delivery of Cisplatin[J]. J Nanopart Res,2014,16(11):2709-2722.
[18]	Zhang W,Guo Z,Huang D,et al. Synergistic Effect of Chemo-Photothermal Therapy Using PEGylated Graphene Oxide[J]. Biomaterials,2011,32(33):8555-8561.

Preparation of Graphene Nano Drug-Loading System and Its Killing Effect on Human Oral Squamous Cell Carcinoma Cells

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 6

Recommended Articles

Metrics

Comments

[1]	ZHOU Chao, SHENG Cheng-Ju, WEN Lin-Lin. Preparation of Imidazolium Salt-based Poly(ionic liquids) Antibacterial Agent and Its Application in Hydrogel Dressing [J]. Chinese Journal of Applied Chemistry, 2021, 38(1): 51-59.
[2]	SHI Lei, YANG Wencong, SHEN Qi, YIN Wei, HUO Zhaohui, SI Liping, LIU Haiyang. Iron Corrole Complexes: DNA-Binding and Anti-tumor Activity [J]. Chinese Journal of Applied Chemistry, 2019, 36(12): 1376-1386.
[3]	WANG Tong, LIU Shi, ZHANG Xiuying. Application of Cisplatin-loaded Thermosensitive Hydrogel in Local Chemotherapy of Cervical Cancer in Mice [J]. Chinese Journal of Applied Chemistry, 2017, 34(10): 1127-1133.
[4]	LI Zhuheng,MA Lina,LIU Dianjun,WANG Zhenxin. Cytotoxicity of Gold Nanoparticles [J]. Chinese Journal of Applied Chemistry, 2016, 33(9): 1009-1016.
[5]	CHU Congbo1, SHAN Yuping2, WANG Hongda2. Application of Quantum Dots in Biological Detection [J]. Chinese Journal of Applied Chemistry, 2014, 31(04): 377-388.
[6]	Ding Daoyuan, Hu Jiehan, Yang Zhiyong. REACTION KINETICS OF CDDP,CBDCA WITH GUANOSINE AND L-Met [J]. Chinese Journal of Applied Chemistry, 1992, 0(3): 114-117.