Chinese Journal of Applied Chemistry ›› 2019, Vol. 36 ›› Issue (5): 564-570.DOI: 10.11944/j.issn.1000-0518.2019.05.180351

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Preparation of Graphene Nano Drug-Loading System and Its Killing Effect on Human Oral Squamous Cell Carcinoma Cells

ZHANG Yajinga,JI Penga,HAN Deminga,ZHAO Lihuia*(),XU Yajuanb*(),LIU Wenshuc   

  1. aCollege of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022,China
    bJilin Cancer Hospital,Changchun 130012,China
    cThe First Hospital of Jilin University,Changchun 130021,China
  • Received:2018-11-06 Accepted:2019-02-28 Published:2019-05-01 Online:2019-05-06
  • Contact: ZHAO Lihui,XU Yajuan
  • Supported by:
    Supported by the Jilin Provincial Science and Technology Agen(No.JJKH20170604KJ)


Graphene oxide was synthesized by chemical redox method. The graphene oxide nanoparticles were prepared by ultrasonic fragmentation and peeling, and functionalized by polyethylene glycol(PEG). The modified graphene oxide was loaded with cisplatin. The graphene nano-drug delivery system was characterized by scanning electron microscopy(SEM), ultraviolet-visible absorption spectroscopy(UV-Vis) and Fourier transform infrared spectroscopy(FTIR). The killing effect of graphene nano-drug delivery system on oral squamous cell carcinoma human oral squamous cell carcinoma(KB) cells was examined by cell survival rate experiment(MTT) method. The results demonstrate that the loading rate of graphene nano-drug-loading system is 42.4%. Polyethylene glycol modified nano graphene oxide can reduce the cytotoxicity of nano graphene oxide and improve its biocompatibility. It has dual killing effect on KB cells, which provides a theoretical basis for the clinical application of nano graphene oxide in cancer treatment.

Key words: nano graphene oxide, cisplatin, cell survival rate experiment, human oral squamous cell carcinoma cells, cytotoxicity