Rapid Determination of Total Amount of Three Estrogens in Milk Powder by Fluorescence Spectrometry

doi:10.11944/j.issn.1000-0518.2018.05.170210

Chinese Journal of Applied Chemistry ›› 2018, Vol. 35 ›› Issue (5): 609-612.DOI: 10.11944/j.issn.1000-0518.2018.05.170210

• Full Papers • Previous Articles

Rapid Determination of Total Amount of Three Estrogens in Milk Powder by Fluorescence Spectrometry

CAI Qihong(),CHEN Xiaoping,LU Jiaxing,CHEN Dengxiu

Key Laboratory of Pharmaceutical Analysis and Laboratory Medicine of Fujian Province University, Pharmaceutical and Medical Technology College,Putian University,Putian,Fujian 351100,China

Received:2017-06-15 Accepted:2017-09-04 Published:2018-04-23 Online:2018-04-23
Contact: CAI Qihong
Supported by:
Supported by the Leading Project of Fujian Provincial Science and Technology Program(No.2015Y0030), the Scientific Research Project of the Education Departerment of Fujian Province(No.JK2014045), the Science and Technology Project of Putian City[No.2013S01(2)], the Scientific Research Project of Putian University(No.2015088)

Abstract

Abstract:

Based on the similarity of the fluorescence properties of three estrogens, a new fluorescence method for direct determination of the total amount of three estrogens(estradiol, estriol, ethinyloestradiol) in milk powder was developed. Taking estriol as the standard reference material, the standard curve method was adopted. The fluorescence intensity of estriol has a linear relationship with the concentration in the range of 1.00~2000 nmol/L and the detection limit of 0.16 nmol/L. The recovery is in the range of 89.11%~98.49%. This method is simple, rapid, highly accurate and sensitive and has been successfully applied to determine the total estrogens in milk powder with satisfactory results.

Key words: estrogens, fluorescence spectrometry, milk powder, estradiol, estriol, ethinyloestradiol

CAI Qihong, CHEN Xiaoping, LU Jiaxing, CHEN Dengxiu. Rapid Determination of Total Amount of Three Estrogens in Milk Powder by Fluorescence Spectrometry[J]. Chinese Journal of Applied Chemistry, 2018, 35(5): 609-612.

References

[1]	Armstrong B,Doll R.Environmental Factors and Cancer Incidenceandmortality in Different Countries, with Special Reference to Dietary Practices[J]. Int J Cancer,1975,15(4):617-631.
[2]	Rose D P,Boyar A P,Wynder E L.International Comparisons of Mortality Rates for Cancer of the Breast, Ovary, Prostate, and Colon, and Per Capita Food Consumption[J]. Cancer,1986,58(11):2363-2371.
[3]	Qin L Q,Wang P Y,Kaneko T,et al. Estrogen: One of the Risk Factors in Milk for Prostate Cancer[J]. Med Hypoth,2004,62(1):133-142.
[4]	YUAN Lijun,XU Zhuangjian,XU Xiangsheng.The Estrogen in Cow's Milk and Its Safety[J]. Int J Inter Med,2007,34(4):712-714(in Chinese). 袁丽君,徐庄剑,许祥生. 牛奶中的雌激素及其安全性[J]. 国际内科学杂志,2007,34(4):712-714.
[5]	Kaipparettu B A,Malik S,Konduri S D,et al. Estrogenmediated Downregulation of CD24 in Breast Cancer Cells[J]. Int J Cancer,2008,123(1):66-72.
[6]	Wang L,Yan H Y,Yang C L,et al. Synthesis of Mimic Molecularly Imprinted Ordered Mesoporous Silica Adsorbent by Thermally Reversible Semicovalent Approach for Pipette-Tip Solid-Phase Extraction-Liquid Chromatography Fluorescence Determination of Estradiol in Milk[J]. J Chromatogr A,2016,1456(1):58-67.
[7]	Xiao X H,Yin Y,Hu Y L,et al. Microwave-Assisted Extraction Coupled with Single Drop Microextraction and High-Performance Column Liquid Chromatography for the Determination of Trace Estrogen Adulterants in Soybean Isoflavone Dietary Supplements[J]. J AOAC Int,2010,93(3):849-854.
[8]	XIAO Fugang,GUO Weiyun,LIU Yan,et al. Determination of Estradiol in Milk Powder by HPLC[J]. Food Res Dev,2011,32(8):81-83(in Chinese). 肖付刚,郭卫芸,刘燕,等. 奶粉中雌二醇的高效液相色谱法检测[J]. 食品研究与开发,2011,32(8):81-83.
[9]	ZHANG Hongying,ZHANG Hong,WANG Shuhui,et al. Determination of Environmental Estrogens in Milk by Gas Chromatography-Mass Spectrometry[J]. Chinese J Health Lab Technol,2007,17(6):1139-1140(in Chinese). 张红英,张宏,王淑惠,等. 气相色谱-质谱法测定牛奶中的8种环境雌激素和内源性雌激素[J]. 中国卫生检验杂志,2009,19(8):1713-1715.
[10]	LIU Yonglin,TIAN Chengxin,YAN Tianwen,et al. Determination of β-Estradiol in Bovine Colostrum by Gas Chromatography-Mass Spectrometry[J]. China Dairy Ind. ,2014,42(7):53/54/55,59(in Chinese). 刘泳麟,田成新,闫天文,等. 气相色谱-质谱法检测牛初乳中β-雌二醇的研究[J]. 中国乳品工业,2014,42(7)):53/54/55,59.
[11]	Tso J,Aga D S.A Systematic Investigation to Optimize Simultaneous Extraction and liquid Chromatography Tandem Mass Spectrometry Analysis of Estrogens and Their Conjugated Metabolites in Milk[J]. J Chromatogr A,2010,1217(29):4784-4795.
[12]	Yang Y,Shao B,Zhang J,et al. Determination of the Residues of 50 Anabolic Hormones in Muscle, Milk and Liver by Very-High-Pressure Liquid Chromatodraphy-Electrospray Ionization Tandem Mass Spectrometry[J]. J Chromatogr B,2009,877(5/6):489-496.

Rapid Determination of Total Amount of Three Estrogens in Milk Powder by Fluorescence Spectrometry

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 7

Recommended Articles

Metrics

Comments

[1]	Lei WU, Ling-Yun LI, Yong-Zhen PENG. Determination of Trace Heavy Metal Elements in Sewage by Total Reflection X-Ray Fluorescence Spectrometry [J]. Chinese Journal of Applied Chemistry, 2023, 40(3): 404-412.
[2]	Tao WANG, Sha LIU, Bao-Lin LIU, Zhi-Xian GAO. Application of Biosensors Based on Aptamers and Antibodies in the Detection of Estradiol [J]. Chinese Journal of Applied Chemistry, 2022, 39(3): 374-390.
[3]	CAI Qihong, WU Yuanyuan, LIN Jiangwei. Rapid Determination of Total Phthalic Acid Esters by Fluorescence Spectrometry [J]. Chinese Journal of Applied Chemistry, 2015, 32(1): 118-122.
[4]	HU Hongyu1,2, ZHANG Lina1,3, CHEN Jiangmin1, ZENG Yanbo1, LI Lei1,2*. Synthesis of Fluorescent Ionic Liquid Probe 1-Butyl-2-phenyl Thiourea Pyridinium Bromide and Its Application in Determination of Mercury Ion(Ⅱ) [J]. Chinese Journal of Applied Chemistry, 2011, 28(12): 1436-1440.
[5]	WANG Rulin*, JIANG Hongyan, QIN Shuzhu, WANG Peng. Inclusions of Lornoxicam by β-Cyclodextrin and Its Derivatives [J]. Chinese Journal of Applied Chemistry, 2011, 28(04): 421-425.
[6]	CAI Xue-Mei1,2, LI Jian-Qing1,2, WEI Yan-Li1, DONG Chuan1*. Interactions Between an Intramolecular Charge Transfer Fluorescence Probe 1-Keto-2-(p-dimethylaminobenzal)-tetrohydronaphthalene and Human Serum Albumin [J]. Chinese Journal of Applied Chemistry, 2010, 27(11): 1313-1317.
[7]	Liu Lihang, Yu Zhiming. Determination of Iron in Milk Powder by Flame Atomic Absorption Spectrometry [J]. Chinese Journal of Applied Chemistry, 1997, 0(3): 111-112.