Chinese Journal of Applied Chemistry ›› 2015, Vol. 32 ›› Issue (11): 1290-1298.DOI: 10.11944/j.issn.1000-0518.2015.11.150145

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Preparation, Characterization and Recognition Behavior of Quercetin-Rutin Bi-template Molecularly Imprinted Polymers

WAGN Susua,ZHANG Yueb,LI Huiab*(),XU Miaomiaoa   

  1. a College of Chemistry and Chemical Engineering
    bKey Laboratory of Plant Resource Conservation and Utilization,Jishou University,Jishou,Hu'nan 416000,China
  • Received:2015-04-23 Accepted:2015-08-20 Online:2015-11-02 Published:2015-11-02
  • Contact: LI Hui
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.21077042), the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hu'nan Province

Abstract:

A bi-templates molecular imprinted polymers(MIPs) was prepared by using quercetin(QT) and rutin(RT) as co-templates. The preparation conditions were optimized. The effects of the molar ratio of two templates and the volume dosage of functional monomer and crosslinker on the adsorption property of MIPs were also studied. Structural characterization of the MIPs was performed by FTIR and SEM. The adsorption dynamics, adsorption isotherm, surface sites distribution and binding selectivity of the MIPs were explored. In addition, the applicability for the MIPs to separate target compound by MIPs solid phase extraction was studied. When the molar ratio of quercetin to rutin is 3:2 and the molar ratio of total templates to functional monomer to cross-linker is 1:8:10, the MIPs obtained possess the highest adsorption capacity toward two templates(47.86 mg/g for quercetin and 60.97 mg/g for rutin). The equilibrium of static adsorption is reached within 3.5 h implying a rapid adsorption dynamics. Scatchard analysis shows that there are four types of binding sites in the polymer matrix, i.e., two types of high affinity binding sites toward two templates and two types of non-selective recognition sites. The relative distribution coefficient(k=Kd(RT)/Kd(QT), Kd=qe/ρe, Kd—distribution coefficient, qe—equilibrium adsorption amount, ρe—equilibrium mass concentration) much higher than 1.0 reveals a high selectivity for the MIPs toward rutin. The highest relative distribution coefficient(6.669) and separation factor(α=qe (RT)/qe(QT))(25.02) are obtained when the concentration of rutin and quercetin in the model mixture is 0.07 and 0.03 mmol/L, respectively. When using acetonitrile, methanol and methanol-acetic acid mixture as effluents in sequence in the MIPs solid phase extraction of crude extract of sophora flower bud, quercetin and rution can be separately extracted with 96.70% and 94.67% recoveries, respectively.

 

Key words: molecularly imprinted polymers, quercetin, rutin, mixed-templates imprinting, molecular recognition

CLC Number: