应用化学 ›› 2020, Vol. 37 ›› Issue (9): 1069-1075.DOI: 10.11944/j.issn.1000-0518.2020.09.200045

• 研究论文 • 上一篇    下一篇

一锅法合成聚乙烯吡咯烷酮修饰的铜纳米团簇用于槲皮素的检测

张申a, 郭玉玉b*   

  1. a太原师范学院化学系 山西 晋中 030619;
    b太原理工大学化学化工学院 太原 030024
  • 收稿日期:2020-02-17 出版日期:2020-09-01 发布日期:2020-09-09
  • 通讯作者: 郭玉玉,讲师; E-mail:guoyuyu@tyut.edu.cn; 研究方向:荧光材料制备
  • 基金资助:
    山西省应用基础研究基金面上自然基金项目(201801D121257)资助

One-Pot Synthesis of Fluorescent Polyvinyl Pyrrolidone-Stabilized Cu Nanoclusters for the Determination of Quercetin

ZHANG Shena, GUO Yuyub*   

  1. aDepartment of Chemistry,Taiyuan Normal University,Jinzhong,Shanxi 030619,China;
    bCollege of Chemistry and Chemical Engineering,Taiyuan University of Technology,Taiyuan 030024,China
  • Received:2020-02-17 Published:2020-09-01 Online:2020-09-09
  • Contact: GUO Yuyu, lecturer; E-mail:guoyuyu@tyut.edu.cn; Research interests:preparation of fluorescent material
  • Supported by:
    Supported by the Shanxi Provincial Applied Fundamental Research Fund Project(No.201801D121257)

摘要: 作为检测槲皮素的有效途径,在荧光法中如何通过简单的方法合成性能优良的荧光探针具有重要的意义。 本文以聚乙烯吡咯烷酮(PVP)为保护剂,抗坏血酸为还原剂,化学还原法合成PVP保护的分散性好、稳定性高、强荧光的铜纳米团簇(PVP-Cu NCs)。 样品表现出良好的水溶性,光稳定性和强离子稳定性。 通过紫外可见光谱(UV-Vis)、分子荧光光谱、透射电子显微镜(TEM)和X射线光电子能谱分析(XPS)对铜纳米团簇的光学性质和结构进行了分析。 结果表明:该铜纳米团簇的最大激发和发射波长分别为366和429 nm,平均粒径大小为2 nm。 基于槲皮素对该铜纳米团簇的猝灭作用,构建了一种可用于检测槲皮素的荧光传感器。 该传感体系检测槲皮素的线性范围为0.1~0.9 μmol/L和15~60 μmol/L,检测限为0.053 μmol/L(S/N=3)。 该传感器对槲皮素的检测具有很高的灵敏度和良好的选择性,可用于实际样品中槲皮素的检测。

关键词: 铜纳米团簇, 聚乙烯吡咯烷酮, 槲皮素, 荧光猝灭

Abstract: As an effective way to detect quercetin, it is important to synthesize fluorescent probe with excellent performance by a simple method. In this work, a facile one-pot approach has been developed to prepare blue-emitting Cu nanoclusters (NCs) using ascorbic acid as a reducing agent and polyvinyl pyrrolidone (PVP) as a capping agent. The PVP-stabilized PVP-Cu NCs display good dispersion, high stability and strong fluorescence. It exhibits good water solubility, excellent photostability and high stability toward high concentration of sodium chloride. The optical performance and structure of PVP-Cu NCs were characterized by ultraviolet-visible (UV-Vis) absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The characterization results show that PVP-Cu NCs have a strong symmetric emission at 429 nm with a maximum excitation at 366 nm. TEM analysis shows that the average diameter of PVP-Cu NCs is 2.0 nm. We construct a fluorescent sensor for the detection of quercetin on the basis of the quenching effect of quercetin on the fluorescence of PVP-Cu NCs. The experimental results show that the quercetin sensing system has a linear range of 0.1~0.9 μmol/L and 15~60 μmol/L with a low detection limit (S/N=3) of 0.053 μmol/L. The sensor has high sensitivity and good selectivity for the detection of quercetin, and can be applied for quercetin detection in real samples.

Key words: copper nanoclusters, polyvinyl pyrrolidone, quercetin, fluorescence quenching