儿茶素-银纳米复合材料的制备及其应用

doi:10.11944/j.issn.1000-0518.2020.09.200076

摘要/Abstract

摘要： 探索了以植物活性成分儿茶素作为还原剂和保护剂一步水热法合成儿茶素-银纳米复合材料,并进一步测试了纳米复合材料的抑菌活性。紫外可见吸收光谱(UV-Vis)和红外光谱(FTIR)测定证明制备得到了儿茶素包裹的银纳米粒子。透射电子显微镜(TEM)和X射线衍射(XRD)结果显示银纳米粒子的平均粒径为22.7 nm,并具有面心立方晶体结构。抑菌活性实验结果表明,儿茶素-银纳米复合粒子对大肠杆菌、金黄色葡萄球菌以及白色念球菌都有很强抑制作用,尤其对白色念球菌的抑制作用最强,其最低抑制浓度(MIC)和最低杀菌浓度(MBC)分别为19.63和39.26 μg/mL。儿茶素-银纳米粒子强抑菌活性可归因于其表面银离子的持续释放,有望应用于长效抑菌制剂产品。

关键词: 儿茶素, 银纳米复合材料, 抑菌活性

Abstract: In this study, we explored a one-step hydrothermal synthesis method to synthesize catechin-silver nanocomposite wherein catechin was acted as the reduction agent and protection agent. The ultrviolet-visible (UV-Vis) and Fourier transform infrared spectroscopy(FTIR) measurements confirm that the obtained nanomaterials are catechin-capped Ag nanoparticles. Transmission electron microscopy(TEM) and X-ray diffraction(XRD) results indicate that the average diameter of the Ag nanoparticles is 22.7 nm, and the Ag nanoparticles have the face centered cubic crystal structure. We further studied the antibacterial activity of catechin-silver nanoparticles. The results of bacteriostatic experiments reveal that the catechin-silver nanoparticles have a strong inhibitory effect on E.coli, S.aureus and C.albicans. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for C.albicans is 19.63 and 39.26 μg/mL, respectively. The inhibitory activity of catechin-silver nanoparticles can be attributed to the continuous release of Ag⁺ from their surface. The catechin-silver nanocomposite is expected to be used in long-acting antibacterial agents.

Key words: catechin, silver nanocomposite, antibacterial activity

邢雅艳, 史宇哲, 邓世贤, 赵柏涵, 刘志国. 儿茶素-银纳米复合材料的制备及其应用[J]. 应用化学, 2020, 37(9): 1062-1068.

XING Yayan, SHI Yuzhe, DENG Shixian, ZHAO Baihan, LIU Zhiguo. Preparation and Application of Catechin-Silver Nanocomposites[J]. Chinese Journal of Applied Chemistry, 2020, 37(9): 1062-1068.

参考文献

[1] YAO Suwei,CAO Yanrui,ZHANG Weiguo. Preparation of Silver Nanoparticles of Different Shapes via Photoreduction Method[J]. Chinese J Appl Chem,2006,23(4):438-440(in Chinese).
姚素薇,曹艳蕊,张卫国. 光还原法制备不同形貌银纳米粒子及其形成机理[J]. 应用化学,2006,23(4):438-440.
[2] Sørensen S N,Lützhøft H H,Rasmussen R,et al. Acute and Chronic Effects from Pulse Exposure of D.magna to Silver and Copper Oxide Nanoparticles[J]. Aquat Toxicol,2016,180:209-217.
[3] Zhang X F,Liu Z G,Shen W,et al. Silver Nanoparticles:Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches[J]. Int J Mol Sci,2016,17(9):1534.
[4] Zhong L J,Hu X,Cao Z M,et al. Aggregation and Dissolution of Engineering Nano Ag and ZnO Pretreated with Natural Organic Matters in the Simulated Lung Biological Fluids[J]. Chemosphere,2019,225:668-677.
[5] Cheng F,Betts J W,Kelly S M,et al. Green Synthesis of Highly Concentrated Aqueous Colloidal Solutions of Large Starch-Stabilised Silver Nanoplatelets[J]. Mater Sci Eng C,2015,46:530-537.
[6] Faria A F,Martinez D S T,Meira S M M,et al. Anti-adhesion and Antibacterial Activity of Silver Nanoparticles Supported on Graphene Oxide Sheets[J]. Colloids Surf B,2014,113:115-124.
[7] Liu Z G,Xing Z M,Zu Y G,et al. Synthesis and Characterization of L-Histidine Capped Silver Nanoparticles[J]. Mater Sci Eng C,2012,32(4):811-816.
[8] Zhou L,Gu H,Wang C,et al. Study on the Synthesis and Surface Enhanced Raman Spectroscopy of Graphene-Based Nanocomposites Decorated with Noble Metal Nanoparticles[J]. Colloids Surf A,2013,430:103-109.
[9] Liu Z G,Wang Y L,Zu Y G,et al. Synthesis of Polyethylenimine(PEI) Functionalized Silver Nanoparticles by a Hydrothermal Method and Their Antibacterial Activity Study[J]. Mater Sci Eng C,2014,42:31-37.
[10] Ramos-Tejada M M,Durán J D G,Ontiveros-Ortega A,et al. Investigation of Alumina/(+)-Catechin System Properties.Part II:ζ-Potential and Surface Free Energy Changes of Alumina[J]. Colloids Surf B,2002,24:309-320.
[11] Deng Z W,Chen M,Wu L M. Novel Method to Fabricate SiO₂/Ag Composite Spheres and Their Catalytic, Surface-Enhanced Raman Scattering Properties[J]. J Phys Chem C,2007,111(31):11692-11698.
[12] Liu J,Sonshine D A,Shervani S,et al. Controlled Release of Biologically Active Silver from Nanosilver Surfaces[J]. ACS Nano,2010,4(11):6903-6913.
[13] Gogoi S K,Gopinath P,Paul A,et al. Green Fluorescent Protein-Expressing Escherichia coli as a Model System for Investigating the Antimicrobial Activities of Silver Nanoparticles[J]. Langmuir,2006,22:9322-9328.
[14] Reidy B,Haase A,Luch A,et al. Mechanisms of Silver Nanoparticle Release, Transformation and Toxicity:A Critical Review of Current Knowledge and Recommendations for Future Studies and Applications[J]. Materials,2013,6:2295-2350.
[15] Biao L H,Tan S N,Wang Y L,et al. Synthesis, Characterization and Antibacterial Study on the Chitosan-Functionalized Ag Nanoparticles[J]. Mater Sci Eng C,2017,76:73-80.
[16] Wu Y Y,Zhang L L,Zhou Y Z,et al. Light-induced ZnO/Ag/rGO Bactericidal Photocatalyst with Synergistic Effect of Sustained Release of Silver Ions and Enhanced Reactive Oxygen Species[J]. Chinese J Catal,2019,40(5):691-704.
[17] Zhang Y Z,Liu X M,Li Z Y,et al. Nano Ag/ZnO Incorporated Hydroxyapatite Composite Coatings:Highly Effective Infection Prevention and Excellent Osteointegration[J]. ACS Appl Mater Interfaces,2018,10(1):1266-1277.