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阳离子型聚乙烯吡咯烷酮的制备及其负载RNA性能

林媚1*,林美玉2,朱炜1,李超萍1,陈何璧1,高凡1,吴珊月1,黄毅楦1   

  1. (1.福建医科大学药学院药物化学系 闽侯 350108；2.复旦大学先进材料实验室 上海 200433)
  • 收稿日期:2013-08-21 修回日期:2013-09-29 出版日期:2014-06-10 发布日期:2014-06-10
  • 通讯作者: 林媚，副教授; Tel:0591-87175951; Fax:0591-22862016; E-mail:linmeichem@126.com; 研究方向：医用与药用高分子材料
  • 基金资助:
    福建省青年人才创新基金资助项目(2008F3049), 福建省大学生创新实验基金资助项目（201310392081）

Preparation of Cationic Polyvinylpyrrolidone and Its Loading Property to RNA

LIN Mei1*, LIN Meiyu2, ZHU Wei1, LI Chaoping1, CHEN Hebi1, GAO Fan1, WU Shanyue1, HUANG Yixuan1   

  1. (1.Department of Medicinal Chemistry,School of Pharmacy,
    Fujian Medical University,Minhou 350108,China;
    2.Laboratory of Advanced Materials,Fudan University,Shanghai 200433,China)
  • Received:2013-08-21 Revised:2013-09-29 Published:2014-06-10 Online:2014-06-10

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1433

被引次数

2 | 1

摘要： 将二甲基二烯丙基氯化铵（DADMAC）与乙烯吡咯烷酮（VP）共聚得到阳离子型的聚乙烯吡咯烷酮（CPVP），并分别合成了聚二甲基二烯丙基氯化铵（PDADMAC）和聚乙烯吡咯烷酮（PVP）均聚物，用粘度法测定了三者的粘均相对分子质量，用红外光谱表征了其结构。 将合成的CPVP与核糖核酸（RNA）以不同比例混合，研究CPVP负载RNA的能力，并与PDADMAC及PVP的负载性能做比较。 结果发现，与PDADMAC及PVP相比，当CPVP与RNA按质量比2∶1混合时，形成的复合体纳米粒的平均粒径约为300 nm，分散度指数为0.0966，粒径分布较窄。

关键词: 阳离子, 聚乙烯吡咯烷酮, 核糖核酸, 纳米粒子

Abstract: Cationic polyvinylpyrrolidone(CPVP) was synthesized by copolymerization of diallyldimethyl ammonium chloride(DADMAC) and vinylpyrrolidone(VP). Polydiallyldimethyl ammonium chloride(PDADMAC) and polyvinylpyrrolidone(PVP) were also synthesized. Their viscosity-average relative molecular masses were measured by viscometry and their structures were characterized by Fourier transform infrared spectroscopy(FTIR). CPVP, PDADMAC and PVP were mixed with ribonucleic acid(RNA) in different ratios in order to measure their loading property. The results indicate that the composite nanoparticles formed by mixing CPVP and RNA with a mass ratio of 2∶1 are about 300 nm with a polydistribution index of 0.0966 and the size distribution of nanoparticles is narrower than those of PVP and PDADMAC.

Key words: cationic, polyvinylpyrrolidone, ribonucleic acid, nanoparticle

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