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基于金纳米粒子的等离子体共振吸收测定阿莫西林

冷飞,李英,甄淑君,李原芳*   

  1. (西南大学化学化工学院,发光与实时分析教育部重点实验室 重庆 400715)
  • 收稿日期:2011-12-30 修回日期:2012-02-15 出版日期:2012-11-10 发布日期:2012-11-10
  • 通讯作者: 李原芳，教授; Tel:023-68254659; Fax:023-68367257; E-mail:liyf@swu.edu.cn; 研究方向：化学与生物药物分析
  • 基金资助:
    国家“重大新药创制”科技重大专项（2010ZX09401-306-1-4）和重庆创新药物孵化基地（2010zx09401-306-1-4）资助项目

Determination of Amoxicillin Based on the Plasmon Resonance of Gold Nanoparticles

LENG Fei, LI Ying, ZHEN Shunjun, LI Yuanfang*   

  1. (Education Ministry Key Laboratory on Luminescence and Real-Time Analysis,College of
    Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)
  • Received:2011-12-30 Revised:2012-02-15 Published:2012-11-10 Online:2012-11-10

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摘要： 在pH=1.89的Britton-Robinson（BR）缓冲溶液中，阿莫西林与氯金酸反应生成金纳米粒子，在537和720 nm产生了特征等离子体共振吸收信号，其537 nm处的吸收强度与阿莫西林浓度在一定范围内呈线性关系，据此建立了基于金纳米粒子的等离子体共振吸收测定阿莫西林的方法。 在优化条件下（pH=1.89，反应温度65 ℃，反应时间40 min），测定阿莫西林的线性范围为2.0×10-6~3.6×10-5 mol/L，检出限为1.3×10-7 mol/L。 该方法用于合成样品中阿莫西林的测定，回收率在90.4%~103.2%之间，RSD小于4.6%，将所建立的方法用于2个厂家生产的阿莫西林胶囊中阿莫西林含量测定，并与HPLC法对比,结果满意。

关键词: 阿莫西林, 金纳米粒子, 等离子体共振

Abstract: In pH=1.89 Briton-Robinson(BR) buffer, HAuCl4 can be reduced by amoxicillin to form gold nanoparticles, resulting in characteristic plasmon resonance absorption signals at 537 nm and 720 nm. The intensity at 537 nm is proportional to the amoxicillin concentration in a certain range, and amoxicillin can be detected based on the plasmon resonance absorption intensities of the gold nanoparticles. Under the optimized conditions(pH=1.89, reaction temperature is 65 ℃, reaction time is 40 min), amoxicillin can be detected in the range of 2.0×10-6~3.6×10-5 mol/L with the detection limit of 1.3×10-7 mol/L. This method has been used in the synthesized samples determination of amoxicillin with the recovery of 90.4%~103.2% and a relative standard deviation of less than 4.6%, then it is applied to the determination of amoxicillin in two commercial capsules with satisfied results compared with HPLC method.

Key words: amoxicillin, gold nanoparticles, plasmon resonance

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