中华医学超声杂志

应用化学 ›› 1994, Vol. 0 ›› Issue (5): 87-90.

• 研究论文 • 上一篇    下一篇

具有谷胱甘肽过氧化物酶活性的催化抗体的研究Ⅰ.谷胱甘肽特征全抗原的合成、表征及性质

朱振齐1, 丁兰1, 罗贵民1, 孙启安1, 杨同书1, 沈家骢2   

  1. 1. 吉林大学酶工程国家重点实验室 长春 130022;
    2. 吉林大学分子光谱和分子结构开放实验室 长春
  • 收稿日期:1993-10-27 修回日期:1994-04-05 出版日期:1994-10-10 发布日期:1994-10-10

Studies on Catalytic Antibody with Glutathione Peroxidase Activity Ⅰ. Synthesis and Property of Glutathione Derivatives as Specific Antigens

Zhu Zhenqi1, Ding Lan1, Luo Guimin1, Sun Qi'an1, Yang Tongshu1, Shen Jiacong2   

  1. 1. The National Laboratory of Enzyme Engineering, Jilin University, Changchun 130023;
    2. Key Laboratory of Molecular Spectrum and Molecular Structure, Jilin University, Changchun
  • Received:1993-10-27 Revised:1994-04-05 Published:1994-10-10 Online:1994-10-10

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摘要： 用2,4-二硝基氯苯保护谷胱甘肽的巯基制出半抗原,再通过戊二醛共价反应使其与牛血清白蛋白表面的氨基结合,经超胶AcA54凝胶层析纯化,制备出有较强免疫原性的谷胱甘肽全抗原。用元素分析、红外光谱及核磁共振表征了半抗原的结构。电泳分析得全抗原分子量平均为87000道尔顿。光谱分析及圆二色谱研究表明其有较强的可见、紫外及荧光特征吸收,且抗原结构的紧密性增强。

关键词: 谷胱甘肽过氧化物酶, 催化抗体, 特征抗原, 合成

Abstract: The sulfhydryl groups of the reduced glutathione (GSH) have been reacted with 2,4-dinitrochlorobenzene to give S-protective glutathione as a hapten, The haptens were crosslinked with the amino groups on the surface of BSA (Bovine Serum Albumin) glutaraldehyde,The covalent conjugates are GSH specific antigens of high immunogenicity.The antigens were found to be homogenious and have a molecular weight of 87000 dalton as determined by gel-electrophoresis method.Spectral and circular dichroism measurements showed that the antigens possess special vis-UV spectra, fluorescence nature with a increased compact of antigen structure.

Key words: glutathione peroxidase, catalytic antibody, specific antigen, preparation

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