中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (12): 1429-1435.DOI: 10.3724/SP.J.1095.2011.00766

• 研究论文 • 上一篇    下一篇

乙酰胆碱酯酶/Nafion/普鲁士蓝修饰玻碳电极的制备及其在有机农药检测中的应用

张梅,汪莉,袁慧珍,刘丽,宋永海*,叶树虹,肖贤平,王菁苒,刘慧萍   

  1. (江西师范大学化学化工学院 南昌 330022)
  • 收稿日期:2010-12-28 修回日期:2011-04-06 出版日期:2011-12-10 发布日期:2011-12-10
  • 通讯作者: 宋永海，副教授; Tel/Fax:0791-8120861; E-mail:lwang@jxnu.edu.cn; 研究方向：表面电化学，生物电化学
  • 基金资助:
    国家自然科学基金（20905032，21065005）江西省自然科学基金（2008GZH0028）江西省教育厅科技项目（GJJ10389）江西师范大学青年成长基金江西师范大学科研启动基金和江西师范大学研究生创新基金(YJS2010060)资助项目

Preparation of Acetylcholinesterase/Nafion/Prussian Blue Modified Glassy Carbon Electrode and Its Application in Pesticide Detection

ZHANG Mei, WANG Li, YUAN Huizhen, LIU Li, SONG Yonghai*, YE Shuhong, XIAO Xianping, WANG Jingran, LIU Huiping   

  1. (College of Chemistry & Chemical Engineering,Jiangxi Normal University,Nanchang 330022)
  • Received:2010-12-28 Revised:2011-04-06 Published:2011-12-10 Online:2011-12-10
  • Contact: Song Yonghai

PDF

1751

被引次数

11

摘要：

制备了乙酰胆碱酯酶/Nafion/普鲁士蓝修饰的玻碳电极，测试了该修饰电极检测有机农药西维因(carbaryl)和敌百虫(trichlorfon)的性能指标。 利用原子力显微镜和电化学技术研究了电极的构造及其对于有机农药检测性能指标的影响。 结果表明，乙酰胆碱酯酶均匀地分散到Nafion/普鲁士蓝修饰的玻碳电极上。 在最优的实验条件下，构筑的修饰电极检测西维因和敌百虫的线性范围分别为0.01~0.5 μmol/L及2.0~10.0 μmol/L和0.02~1.0 μmol/L及2.0~8.0 μmol/L，检出限分别为5.0和10.0 nmol/L。 并对模拟的实际样品进行了检测，发现该方法有较高的检测灵敏度、较好的重复性和抗干扰性。

关键词: 西维因, 敌百虫, 普鲁士蓝, Nafion, 乙酰胆碱酯酶, 电化学

Abstract:

This paper reports the preparation of an acetylcholinesterase(AChE)/Nafion/Prussian blue(PB)/glass carbon electrode(GCE) and the electrochemical detection of pesticides such as carbaryl and trichlorfon. Atomic force microscopy and electrochemical technique were used to investigate the construction of the modified electrode and its effects on pesticide detection. Those results indicated that AChE dispersed uniformly in Nafion/PB/GCE. The AChE/Nafion/PB/GCE constructed under optimal conditions showed a good linear relationship between the peak current and the concentration of pesticide in the range of 0.01~0.5 μmol/L & 2.0~10.0 μmol/L for carbaryl and 0.02~1.0 μmol/L & 2.0~8.0 μmol/L for trichlorfon, respectively. The limit of detection is 5.0 nmol/L for carbaryl and 10.0 nmol/L for trichlorfon, respectively. Furthermore the modified electrode was applied in the detection of analogous practical samples. It was found that the as-prepared sensor had high detection sensitivity, good reproducibility and anti-interference.

Key words: Carbaryl, Trichlorfon, Prussian blue, Nafion, Acetylcholinesterase, Electrochemistry

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