中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (03): 338-342.DOI: 10.3724/SP.J.1095.2011.00295

• 研究论文 • 上一篇    下一篇

氯霉素分子印迹聚合膜电极的制备及氯霉素检测

张丽君,陆天虹,李时银,杜江燕*   

  1. (江苏省生物功能材料重点实验室,南京师范大学化学与环境科学学院 南京  210046)
  • 收稿日期:2010-05-18 修回日期:2010-07-08 出版日期:2011-03-10 发布日期:2011-03-10
  • 通讯作者: 杜江燕，教授; Tel:025-85891651; Fax:025-85891767; E-mail:dujiangyan@njnu.edu.cn; 研究方向：生物电化学

Fabrication and Application of CAP-MIP-OAP Film Electrode for Chloramphenical Detection

ZHANG Li-Jun, LU Tian-Hong, LI Shi-Yin, DU Jiang-Yan*   

  1. (Jiangsu Key Laboratory of Bio-functional Materials,College of Chemistry and
    Environmental Science,Nanjing Normal University,Nanjing 210046)
  • Received:2010-05-18 Revised:2010-07-08 Published:2011-03-10 Online:2011-03-10

PDF

1270

被引次数

7

摘要：

以邻氨基酚(OAP)为功能单体，氯霉素(CAP)为模板分子，用电化学聚合的方法在Pt上合成了CAP分子印迹（MIP）OAP膜(CAP-MIP-OAP/Pt)电极，通过扫描电子显微镜和电化学技术对聚合膜的结构和性能进行了表征。 结果表明，该膜电极对CAP检测有较好的选择性和灵敏度，CAP检测的线性范围为4.33×10-8～3.09×10-6 mol/L，检出限为2.5×10-8 mol/L。

关键词: 电化学聚合, 氯霉素, 邻氨基酚, 分子印迹聚合膜, 电化学检测

Abstract:

The chloramphenicol(CAP) molecule-imprinted polymer(MIP) of o-aminophenol(OAP) on Pt(CAP-MIP-OAP/Pt) electrode was prepared via an electrochemical polymerization method using OAP as the functional monomer and CAP as the template molecule. This preparation method is simple. The as-prepared membrane is uniform and stable. The performances and configurations of the CAP-MIP-OAP/Pt and MIP-OAP/Pt electrodes were investigated using scanning electron microscope(SEM) and electrochemical techniques. The results illustrated that the MIP-OAP/Pt electrode exhibited improved selectivity and sensitivity for the CAP detection. A linear region of the CAP detection is in the range of 4.33×10-8～3.09×10-6 mol/L. The detection limit is 2.5×10-8 mol/L. Therefore, the MIP-OAP/Pt electrode shows potential application for the enrichment and detection of CAP in the environmental water sample.

Key words: electrochemical polymerization, chloramphenicol, o-aminophenol, molecular imprinted polymer film, electrochemical detection

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